A method was developed for the determination of Aroclor 1254 in water samples using polystyrene-divinylbenzene(PS-DVB)solid-phase extraction(SPE)and gas chromatography(GC).The PS-DVB sorbent demonstrated good recoveries of Aroclor 1254 when sulfuric acid(1:1,v:v)was added to the samples.Sample quantification was performed using gas chroma-tography with a parallel column and dual electron capture detector.Factors in extraction method procedures were optimized,including sample pH,elution solvent,eluting volume and breakthrough volume.With the 1000 ml sample acidified to pH 1.0,a 6.0 ml n-hexane/ethyl acetate(1:1,v:v)elution solvent,and gas chromatographic determination,the recoveries of Aroclor 1254 in spiked water samples ranged from 90.6 to 104%.Across testing days,repeatability ranged from 4.7 to 7.6%.The method detection limit was 0.053μg/L.PS-DVB exhibited a broader pH stability range,satisfactory recoveries,and good repeatability for Aroclor 1254 extraction.The results indicate that these methods can be used to analyze trace amounts of Aroclor 1254,and represent a new analytical approach for determining polychlorinated biphenyls in water samples.
Fei ShenYan-Juan XuYe WangPei-Yu ZhuXiu-Li DaiJing ChenQian Zou
Objective:To evaluate the dose and duration dependent cytotoxicity of aroclor 1254 on mice testis.Methods: The study tests the hypothesis that in vivo exposure of very low dose aroclor 1254, comparable to that of possible human exposure from different environmental sources, will provoke dose and duration dependent histological damage in the mice testis. Male mice were orally administered with the two doses 0.1 and 1 mg/kg b.w /d. of aroclor 1254 for 7, 14, 21 and 28 days.Results:Results showed the degenerative changes in the testis of mice, namely, atrophied seminiferous tubules, expanded space in interstitial and necrosis in the germinal epithelial cells of seminiferous tubules, of the seminiferous tubules and deceleration of spermatogenesis.Conclusions: Therefore, the results of the present study suggested that the sub-acute exposure of very low doses of aroclor 1254, can subsequently mediate the cytoskeleton dysfunction in the testis of mice.
