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河北省自然科学基金(303600)

作品数:2 被引量:16H指数:2
相关作者:张继澍周厚成关军锋樊秀彩更多>>
相关机构:中国农业科学院郑州果树研究所西北农林科技大学河北省农林科学院更多>>
发文基金:河北省自然科学基金国家自然科学基金更多>>
相关领域:农业科学生物学更多>>

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外源乙烯对桃果实微粒体膜Ca^(2+)-ATPase活性和膜脂过氧化水平的影响被引量:14
2005年
以'迎庆'桃果实为材料,研究了经外源乙烯(50μL/L)处理12h后,在常温(25℃)下贮藏过程中,果实微粒体膜Ca2+-ATPase活性和膜脂过氧化水平的变化。结果表明:外源乙烯促进果实内源乙烯的生成,刺激膜Ca2+-ATPase活性先上升而后下降,同时加速超氧自由基的产生,提高膜脂过氧化产物丙二醛含量,增强了膜脂过氧化作用;钙调素拮抗剂三氟拉嗪(TFP)和钙通道阻塞剂异博定(VER)均在一定程度上抑制乙烯诱导的上述效应,这表明细胞内Ca2+和CaM参与了外源乙烯反应。
樊秀彩关军锋周厚成张继澍
关键词:乙烯膜脂过氧化
The Relationship Between Senescence and Ca^(2+)-ATPase Activity of Microsomal Membrane and Lipid Peroxidation in Harvested Peach Fruit被引量:2
2006年
Peach fruit easily soften and have a short storage time at normal temperature. In this study, peach fruit (Prunus persica sieb et Zucc cv. Yingqing) were picked and stored at 25 and 4°C to investigate the senescence in correlation with Ca2+- ATPase activity of microsomal membrane and lipid peroxidation during ripening and senescence. In comparison with that stored at 25°C, the fruit stored at 4°C exhibited a higher flesh firmness, lower respiration rate, and generated the late bigger peak value of Ca2+-ATPase activity as well as maintained the higher activity of the enzyme. Meanwhile, the lower levels of super oxygen radical (O2·–) production and content of malondialdehyde (MDA), a product of membrane lipid peroxidation were observed. Sodium orthovanadate (SO) and erythrosin B (EB), as Ca2+-ATPase inhibitors, could stimulate the respiration rate. The results suggested that the slower senescence rate of peach fruit was closely related to the higher peak value and longer duration of Ca2+-ATPase activity in microsomal membrane, with the slighter membrane lipid peroxidation and lower O2·– production rate.
GUAN Jun-fengFAN Xiu-caiDOU Shi-juanZHANG Ji-shuLI Guang-min
关键词:桃子过氧化反应
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