Xing Li Zhang~(a,c,d),Yang Yang Liu~b,Jian He Wei~(b,c,*),Yun Yang~b, Zheng Zhang~(b,c),Jun Qing Huang~c,Huai Qiong Chen~c,Yu Jun Liu~a (a College of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083,China b Hainan Branch of Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences,Wanning 571533,China c Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China d School of Food & Biological Engineering,Shandong Institute of Light Industry,Jinan,250353,China)
Aquilaria sinensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional Chinese medicine, perfume and incense.Coronatine-insensitive protein 1(COI1) acts as a receptor in jasmonate(JA) signaling pathway, and regulates the expression of JA-responsive genes in plant defense. However, little is known about the COI1 gene in A. sinensis. Here, based on the transcriptome data, a full-length cDNA sequence of COI1(termed as AsCOI1) was firstly cloned by RT–PCR and rapid-amplification of cDNA ends(RACE) strategies.AsCOI1 is 2330 bp in length(Gen Bank accession No. KM189194), and contains a complete open frame(ORF) of 1839 bp. The deduced protein was composed of 612 amino acids, with a predicted molecular weight of 68.93 k Da and an isoelectric point of 6.56, and was predicted to possess F-box and LRRs domains. Combining bioinformatics prediction with subcellular localization experiment analysis, AsCOI1 was appeared to locate in nucleus. AsCOI1 gene was highly expressed in roots and stems, the major organs of agarwood formation. Methyl jasmonate(Me JA), mechanical wounding and heat stress could significantly induce the expression level of AsCOI1 gene. AsCOI1 is an early wound-responsive gene, and it likely plays some role in agarwood formation.
