[Objective] The aim was to make bioinformatics analysis on MSTN gene in swine. [Method] In the research, coding sequences （CDS） of MSTN gene in swine, rat, mouse, dog, sheep, goat, cattle, chimpanzee, human, horse, chicken and zebra fish were loaded to Megalign of DNAstar for analysis of phyletic evolution. In addi- tion, we also made analysis on basic information, restriction map, secondary struc- ture of coding protein, signal peptide, transmembrane domain and protein subcellular localization. [Result] Closely related to rat, mouse, dog, sheep, goat, cattle, chim- panzee, human and horse, MSTN genes in swine included many enzyme cutting sites and the encoding protein was unstable in hydrophobicity. The molecular weight was 42 791.3 u, isoelectric point was 6.98, and the gene included 375 amino acid residues. In addition, the secondary structure of protein contained 20.53% of c^-Helix, 4% of 15-Turn, 53.07% of Coil, 22.4% of extenden strand and one transmembrane domain. The extracellular protein was probably being signal peptide. [Conclusion] The research provided references for further study on MSTN genes of swine.
