Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary metabolites.The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology.However,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced microbes.This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.
Space flight experiments have suggested that microgravity can affect cellular processes in microorganisms.To simulate the microgravity environment on earth,several models have been developed and applied to examine the effect of microgravity on secondary metabolism.In this paper,studies of effects of space flight on secondary metabolism are exemplified and reviewed along with the advantages and disadvantages of the current models used for simulating microgravity.This discussion is both significant and timely to researchers considering the use of simulated microgravity or space flight to explore effects of weightlessness on secondary metabolism.
