To investigate the effects of silver nanoparticles(Ag NPs)and low temperature double-pressure on the wastewater treatment efficacy and the microbial community structure of constructed wetlands,a pilot-scale vertical flow constructed wetland was set up to treat synthetic wastewater under laboratory conditions.By measuring the effluent concentration of ammonia nitrogen(NH_(4)^(+)-N),total nitrogen(TN),total phosphorus(TP),chemical oxygen demand(COD),and the diversity,richness,and community structure of microorganisms of the upper and lower soil layers in the wetland,the nutrient removal effect of the constructed wetland and the changes in the microflora of the soil layer were studied.The results reveal that the correlation coefficients between the removal rates of TN and NH_(4)^(+)-N and the temperature are 0.463 and 0.692,respectively,indicating a significant positive correlation.From the warm to the cold season,both the diversity and richness of microorganisms in the lower soil layer of wetlands are inhibited under the double-pressure of Ag NPs and low temperature,and the abundances of the denitrogenation functional bacteria such as Candidatus nitrososphaera,Sulfuritalea,Anaeromyxobacter,Candidatus solibacter,Nitrospira,and Zoogloea are altered.Low temperature and Ag NPs exposure can thus affect the wastewater treatment performance of constructed wetlands,possibly because of the seasonal changes of the microflora.
In order to explore the nitrogen removal process in constructed wetlands(CW s),the moisture,ammonia nitrogen(NH4+-N),nitrate nitrogen(NO3"-N)and nitrification intensity in three wetland plant rhizosphere soils(Acorns calamus,Typha orientalis,Iris pseudacorus)were investigated at a relatively normal temperature range of15to25The relative abundance of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)were also achieved using fluorescence in situ hybridization(FISH).It is found that T.orientalis achieves the highest nitrification intensity of2.03m g(h?kg)while the second is I.pseudacorrs(1.74m g/(h?kg)),and followed by A.calamus(1.65m g/(h?kg))throughout the experiment.FISH reveals that the abundance of bacteria(1010g_1wet soil)is higher than that of archaea(109g_1wet soil),and AOBare the dominant bacteria in the ammonia oxidation process.The abundance of AOB in te rhizosphere soils from high to low T.orientalis(1.88x1010g"1),I pseudacorus(1.23x1010g1),A.calamus(5.07x109g"1)while the abundance of AOA from high to low ae I.pseudacorus(4.00x109g1),A.calamus(3.52x109g"1),T.orientalis(3.48x109g"1).The study provides valuable evidence of plant selection for nitrogen removal in CWs.
Yan ChunniHuang JuanYang SisiCao ChongPeng ChengLi Runqing
