A method based on the XYZLMS interim connection space is proposed to accurately acquire the multi-spectral images by digital still cameras. The XYZLMS values are firstly predicted from RGB values by polynomial model with local training samples and then spectral reflectance is constructed from XYZLMS values by pseudo-inverse method. An experiment is implemented for multi-spectral image acquisition based on a commercial digital still camera. The results indicate that multi-spectral images can be accurately acquired except the very dark colors.