Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patients were determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.Results:RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperative survival(HR=1.313,95%CI=1.128~1.537,P<0.001).Conclusion:High expression of RNF187 in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.
Rong TangLin-Mei ZhengZhen-Sheng ZhangJin-Cai WuJin-Fang ZhengLiangChenYi-Chao DingPing-Ping ChenJia-Chao ZhangYong-Chao Zeng
Objective: To investigate the effect of hepatic differentiation of human bone marrow mesenchymal stem cells (HBMSCs) induced in vitro and transplanted into rats with liver failure via portal vein, and observe the changes of liver function and pathological tissue. Method:After passage to the 6th generation in vitro, the hepatic differentiation was induced by HGFand EGF inducible factors. CCL4 acute liver failure model in rats were established, and randomly divided into 5 groups transplanted with differentiated stem cells via portal vein. These five groups included HGF-differentiated HBMSCs transplantation, EGF-differentiated HBMSCs transplantation, EGF+HGF-differentiated HBMSCs transplantation, non-differentiated HBMSCs transplantation, and non-HBMSCs transplantation. Liver function and pathological changes were detected. Results: Rats models survival, serum albumin, aminotransferase and coagulation indexes were observed at 12 h, 72 h, 7 d, 1 month and 2 months after treatment. The results showed that the survival and albumin, aminotransferase and coagulation function of rats were improved significantly after treatment in HGF-differentiated, EGF-differentiated, EGF+HGF-differentiated and non-differentiated transplantation groups, compared tothe non-HBMSCstransplantation group(P<0.05), while no significance was observed in above four groups(P>0.05).Pathological changes was ameliorated in the liver of rat models in HGF-, EGF-, EGF+HGF- and non-differentiated transplantation groups, compared to the non-HBMSCs transplantation group. Conclusion: Liver-differentiated BMSCs transplanted into rats with liver failure could effectively improve liver function and survival rate.
Yang-Gang YanJin-Cai WuJia-Cheng ChenDa-Feng XuCheng ChenXing LiSheng-Yi TanZhuo-Ri Li
