您的位置: 专家智库 > >

国家自然科学基金(30970172)

作品数:3 被引量:7H指数:2
相关作者:吴昌银彭强陈国兴邱咏更多>>
相关机构:华中农业大学更多>>
发文基金:国家自然科学基金国家高技术研究发展计划更多>>
相关领域:农业科学生物学更多>>

文献类型

  • 3篇中文期刊文章

领域

  • 3篇农业科学
  • 1篇生物学

主题

  • 1篇水稻
  • 1篇穗期
  • 1篇特异
  • 1篇特异表达
  • 1篇启动子
  • 1篇启动子驱动
  • 1篇维管组织
  • 1篇酵母
  • 1篇酵母双杂交
  • 1篇PERSPE...
  • 1篇REVIEW
  • 1篇SMART技...
  • 1篇CDNA文库
  • 1篇IN_CHI...
  • 1篇抽穗
  • 1篇抽穗期
  • 1篇CONTRI...
  • 1篇EFFORT
  • 1篇RICE

机构

  • 2篇华中农业大学

作者

  • 2篇吴昌银
  • 1篇邱咏
  • 1篇陈国兴
  • 1篇彭强

传媒

  • 1篇华中农业大学...
  • 1篇分子植物育种
  • 1篇Journa...

年份

  • 2篇2012
  • 1篇2010
3 条 记 录,以下是 1-3
排序方式:
维管组织特异表达启动子驱动RID1调控水稻抽穗期
2010年
水稻基因RID1突变后表现为"不开花"的表型,且RID1特异在幼叶中表达。本研究利用启动子RPP16,构建表达载体RPP16::RID1,转化rid1突变体,得到了19株转基因阳性植株,其中有9株表现为恢复抽穗,表明在维管组织中特异表达RID1可以恢复rid1突变体表型。RT-PCR检测结果显示,转基因单株中抽穗期基因Ehd1、Hd3a和RFT1的表达量与RID1表达量呈正相关,且RID1表达量高的转基因单株相应抽穗也比较早。
邱咏陈国兴吴昌银
关键词:水稻抽穗期
水稻抽穗期基因酵母双杂交cDNA文库的构建及分析被引量:3
2012年
为研究水稻开花调控的分子网络,构建了水稻品种中花11幼穗分化前倒二叶叶片的酵母双杂交cDNA文库。制备了高质量的总RNA,用Oligo(dT)引物反转录合成cDNA第一链和Long Distance PCR合成双链cD-NA,通过SMART同源重组交换技术在酵母菌株AH109中建立酵母双杂交所需的水稻倒二叶叶片cDNA文库。文库质量检测结果表明:cDNA文库的转化率为1.178×106/3μg pGADT7-Rec,文库滴度为2.65×108 cfu/mL,重组率为94.7%,插入片段长度为350~2 000bp。该文库可用来筛选水稻抽穗期基因的互作蛋白。
彭强吴昌银
关键词:酵母双杂交SMART技术CDNA文库
Effort and Contribution of T-DNA Insertion Mutant Library for Rice Functional Genomics Research in China:Review and Perspective被引量:4
2012年
With the completion of the rice (Oryza sativa L.) genome-sequencing project, the rice research community proposed to characterize the func- tion of every predicted gene in rice by 2020. One of the most effective and high-throughput strategies for studying gene function is to employ genetic mutations induced by insertion elements such as T-DNA or transposons. Since 1999, with support from the Ministry of Science and Technology of China for Rice Functional Genomics Programs, large-scale T-DNA insertion mutant populations have been generated in Huazhong Agricultural University, the Chinese Academy of Sciences and the Chinese Academy of Agricultural Sciences. Currently, a total of 372,346 mutant lines have been generated, and 58,226 T-DNA or Tos17 flanking sequence tags have been isolated. Using these mutant resources, more than 40 genes with potential applications in rice breeding have already been identified. These include genes involved in biotic or abiotic stress responses, nutrient metabolism, pollen development, and plant architecture. The functional analysis of these genes will not only deepen our understanding of the fundamental biological questions in rice, but will also offer valuable gene resources for developing Green Super Rice that is high-yielding with few inputs even under the poor growth conditions of many regions of Africa and Asia.
Yuxiao ChangTuan LongChangyin Wu
关键词:RICE
共1页<1>
聚类工具0