目的探讨原发性胆囊癌患者是否存在血管生成拟态(VM)及其 VM 存在的临床意义。方法收集1994年1月至2000年12月期间同济大学附属同济医院收治的74例经手术切除和病理确诊的原发性胆囊癌患者病理标本及相关临床病理参数和预后指标;应用 HE 染色、CD_(31)和 PAS双重染色,观察胆囊癌患者是否存在 VM,并作上述相关参数的单因素分析、Kaplan-Meier 生存比较和Cox 风险模型分析。结果在74例胆囊癌患者中发现13.5%(10/74)存在 VM。胆囊癌 VM 与患者性别、年龄、肿瘤部位、瘤体大小、分化程度、Nevin 分期、浸润深度、局部淋巴转移无关;但与组织类型(x^2=10.241,P=0.017)、肝转移(x^2=11.904,P=0.001)和生存期(x^2=5.7221,P=0.0168)有关。Cox 分析表明,VM(F=24.0508,P=0.0001,相对危险度=11.868)和浸润深度、淋巴结转移、肝转移、手术方式(姑息手术)一样,是影响胆囊癌患者预后的独立的危险因素。结论人原发性胆囊癌存在VM,有 VM 存在的胆囊癌患者,易发生血行转移,预后差。
Objective To explore if vasculogenic mimicry (VM) exists in human primary gallbladder carcinomas and evaluate the correlation between the VM and expression of vascular epithelial growth factor (VEGF) in gallbladder carcinomas and its significance. MethodsSeventy-four carcinomas, 10 adenomas and 10 chronic inflammatory lesions of the gallbladder underwent operation and confirmed histopathologically were studied. Clinical-pathological data and survival of each patient with gallbladder carcinoma were recorded and followed-up. VM in human gallbladder carcinomas was observed under light microscope by HE staining, CD31 and PAS staining; the expression of VEGF proteins in each paraffin section of each patient in vivo was determined by Envision method of immunohistochemistry; the correlation among the VM, VEGF expression and their clinical significance in the patients with gallbladder carcinomas were analyzed and compared by Kaplan-Meier actuarial survival curves and Cox multiple factors. Results①13.5% (10/74) of human gallbladder carcinomas were found to contain VM, namely intratumoral, tumor cell-lined extracellular matrix (ECM)-rich, PAS-positive and vasculogenic-like network patterns. VM was associated with histological type (χ2=10.241,P=0.017), hepatic metastasis (χ2=4.238,P=0.042) and poor overall survival (χ2=5.722 1,P=0.016). ②Expression of VEGF was increased significantly in carcinomas with or without VM than adenomas and inflammatory lesions of the gallbladder (P<0.000 1) in vivo; VEGF expression in the gallbladder carcinomas without VM was increased significantly than that with VM (P=0.018 2). ③There is positive correlation between expression of VEGF and the gallbladder carcinomas without VM in the cases of Nevin stage (P=0.003 5), invasion depth (P=0.005 9), liver metastases (P=0.037 3) and lymph node metastases (P=0.000 1), the same correlation was only observed between expression of VEGF and the gallbladder carcinomas with VM in the cases of liver metastases (P=0.032 3). When being compared the non
