Higher fullerenes(C84,C90,C92,C94 and C96) were successfully isolated from the Soxhlet extract of the synthetical "Graphote Smokes" soot(GS sample) by using a big Cosmosil Buckysep(Phenomenex) column(250 mm × 10 mm) with a large injection.The fractions isolated have been determined by high performance liquid chro-matography(HPLC) and laser desorption mass spectrometry(LDMS).It is found that there are different fullerenes molecules in different fractions with retention time.The result indicates that fullerenes do exist in GS samples.Also,it excludes the suspicion to some extent that fullerene molecules might be generated by the laser desorption process in the LDMS.In addition,it also provides the experimental basis for the study of natural higher fullerenes and might be helpful to figure out the question if higher fullerenes do exist in the natural samples.
