Sodium dodecyl sulfate(SDS)/dodecyl triethyl ammonium bromide(DEAB)mixed micelles can transform to vesicles only when the temperature was higher than a critical value.In this study,by using UV-vis spectrometer and fluorescence microscopy,we found that oligo(rU)15 can decrease the critical temperature to a much lower value and hence,induce vesicle formation.Considering the importance of RNAi in gene therapy and the biohazards of viral vector,this finding should provide some helpful information for the transfer of RNA using nonviral vector in gene therapy.
囊泡是由密闭双分子层所形成的球形或椭球形结构。将60 IU FSH分别溶于10 mmol/L和15 mmol/L的囊泡液中对母兔一次性肌肉注射进行超数排卵处理,对照组将同量FSH溶于生理盐水中分次连续注射。结果表明:2个试验组和对照组母兔卵巢上各级卵泡数均差异不显著(P>0.05)且试验Ⅰ组卵子回收数(37.83±7.17)与试验Ⅱ组卵子回收数(37.50±8.34)均高于对照组卵子回收数(34.67±10.03),组间差异不显著(P>0.05)。由此可见,囊泡对FSH具有包埋和缓释的作用,可延长FSH在动物体内的半衰期,可以用于家兔的超数排卵。
