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国家自然科学基金(20973015)

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相关作者:赵新生尹延东支泽勇周晓雪刘鹏程更多>>
相关机构:北京大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划更多>>
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最大熵法分析寡聚核苷酸链内碰撞的荧光相关光谱(英文)被引量:1
2010年
准确地由荧光相关光谱(FCS)的实验数据提取动力学信息一直是一个挑战.本文对比了三种主要的方法:依赖于模型的多指数函数法,经验的拓展指数函数法和不依赖于模型的最大熵法.多指数函数法的物理意义直接但在复杂体系中难以应用和解释.拓展指数函数法简单易行但其物理意义含混不清.最大熵法不依赖于具体的物理模型但拟合结果对实验噪音很敏感.经研究我们发现一个好的选择是将最大熵法和多指数函数法结合在一起使用.对寡聚核苷酸链内碰撞荧光相关光谱的研究发现,在单链DNA中可以形成碱基对时,有两个并行的链内碰撞反应.以前的拓展指数函数法分析则不能提供这样的信息.我们建议在荧光相关光谱研究中审慎地使用最大熵法.
尹延东周晓雪赵新生
关键词:寡聚核苷酸光致电子转移
Single molecule study on DNA/RNA modification
<正>Why and how do twins develop into individuals of different identities, although they carry almost identical...
赵新生
关键词:MODIFICATION
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PET and FCS study in kinetics of H/ACA RNP
<正>H/ACA RNP(ribonucleoprotein complex) containing H/ACA RNA,Cbf5,Nop10,L7Ae,and Gar1 can guide site-specific ...
Xin-Xing Yang~(§1),Jing-Qi Duan~(§2),Ye-Ke Qiong~2,Xin-Sheng Zhao~(*1) 1 State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing,100871, China 2 National Institute of Biological Sciences,Beijing 102206,China§The authors contributed equally to this work
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Silver Enhancement-Aptamer-Gold Nanoparticles Aggregation Based Assay for Simple and Sensitive Detection of Adenosine
<正>Aptamers are oligonucleic acid or peptide molecules that bind to wide range specific targets from metal ion...
Zhaofang Liu,Jing Ge,and Xin Sheng Zhao~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
关键词:APTAMER
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Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oligonucleotide Intra-chain Collision
<正>It has been a challenge to accurately extract dynamic information from experimental fluorescence correlatio...
YIN Yan-Dong,ZHOU Xiao-Xue,ZHAO Xin-Sheng~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,P.R.China
关键词:OLIGONUCLEOTIDES
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Probing Knot Location and Size in Denatured Knotted Protein by Single Molecule Spectroscopy
<正>An increasing number of proteins are being found to contain a knot formed by the polypeptide chain. Why and...
Peng WangLijiang YangPengcheng LiuYi Qin GaoXin Sheng Zhao
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Qualitative and Quantitative Protein Detection in Undiluted Serum with Gold Nanoparticles
<正>One of the key challenges in bioanalytical chemistry is the detection of disease-related protein and metabo...
Jing Ge,Xin Sheng Zhao~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
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Enzyme Dynamics of AIKB
A1KB is a kind of enzyme that repairs MethyNA.The phenomenon that double strand DNA(dsDNA) will be contorted w...
Xun Li,Xin Sheng Zhao~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
关键词:DYNAMICENZYME
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Intermediates in the GdmCl-induced equilibrium unfolding of knotted tRNA Methyltransferase from E.Coli.
<正>tRNA(guanosine-1) methyltransferase(TrmD) from E.Coli.,which methylates G 37 of tRNAs containing the sequen...
Pengcheng Liu,Peng Wang,Ronghua Sun,Xin Sheng Zhao~* Beijing National Laboratory for Molecular Sciences,State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
关键词:INTERMEDIATES
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从化学到生物学
<正>化学生物学是一个化学与生物学交叉的新兴学科。其特点之一是将化学的最新成果(技术、方法、理论)应用到生命现象的研究当中。到20世纪末,化学又有了崭新的面貌。例如,分子反应动力学的发展将物理化学推向一个新的高峰。如何将...
赵新生
关键词:单分子探测物理化学化学生物学
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