Cervus sichuanicus is a species of sika deer(Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve(TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After Gene Scan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer(Cervus hortulorum) released in TNR.
Nonlethal human recreational activities have been confirmed to have negative effect on wild animals in a number of ways,including changes in behaviors,avoidance of suitable habitats and declines in breeding success.Studies on the anti-disturbance mechanism of wild animals to human disturbance can provide valuable knowledge to the management of wild animals and the evolutionary mechanisms of behavioral adaptation to their habitats.To evaluate how blue sheep(Pseudois nayaur) would react to nonlethal human recreational disturbance,we studied their anti-disturbance strategy towards human disturbance in Suyukou National Forest Park(SNFP),Helan Mountains,Yinchuan of Ningxia Hui Autonomous Region.Using multinomial logistic regression models(MLRMs),we sought to answer two questions:(1) which kind of human recreational behavior would evoke the most serious anti-disturbance behaviors in blue sheep;and(2) how would various ecological factors influence the anti-disturbance strategy of blue sheep to human recreational disturbance? We collected 10 habitat and population variables and evaluated three kinds of reaction of blue sheep-no response,vigilance and flight.A total of 921 observations qualified to enter MLRMs.We found that habitat type(HT),gender(GEN),head direction(HD),visibility index(VI),and disturbance source(DS) were the five variables that significantly influenced the intensity of reactions of blue sheep.Blue sheep were more alert to tourists than to vehicles,and roads were the habitat type that caused the most intensive reaction of alertness where human disturbance was the highest.Females were more vigilant than males.Blue sheep might feel safer when staying in open habitat,and taking a front head direction provided them with the highest vigilance.Based on these results,we present suggestions to SNFP for the management of ecotourism and blue sheep conservation.
