Excessive activation of macrophages is implicated in various inflammatory injuries.Salidroside (Sal),one of the main bioactive components of Rhodiola Sachalinensis,has been reported to possess anti-inflammatory activities.This study aimed to examine the effect of Sal on the activation of macrophages and the possible mechanism.The lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were established.The changes in the inflammatory profiles of THP-1-derived macrophages were determined.The results showed that Sal significantly decreased the expression of inducible nitric oxide synthase (iNOS),cyclooxygenase-2 (COX2),interleukin-1beta (IL-1β),interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) at both mRNA and protein levels in THP-1-derived macrophages,and the effect was dose-depedent.Moreover,NF-κB activation was significantly suppressed and the phosphorylation of ERK,p38 and JNK was substantially down-regulated after Sal treatment.The findings suggested that Sal can suppress the activation of LPS-stimulated PMA-differetiated THP-1 cells,as evidenced by the decreased expression of iNOS,COX2,IL-1β,IL-6 and TNF-α,and the mechanism involves the inhibition of NF-κB activation and the phosphorylation of the MAPK signal pathway.
