Background:Early pregnancy failure has a profound impact on both human reproductive health and animal production.2/3 pregnancy failures occur during the peri-implantation period;however,the underlying mechanism(s)remains unclear.Well-organized modification of the endometrium to a receptive state is critical to establish pregnancy.Aberrant endometrial modification during implantation is thought to be largely responsible for early pregnancy loss.Result:In this study,using well-managed recipient ewes that received embryo transfer as model,we compared the endometrial proteome between pregnant and non-pregnant ewes during implantation period.After embryo transfer,recipients were assigned as pregnant or non-pregnant ewes according to the presence or absence of an elongated conceptus at Day 17 of pregnancy.By comparing the endometrial proteomic profiles between pregnant and non-pregnant ewes,we identified 94 and 257 differentially expressed proteins(DEPs) in the endometrial caruncular and intercaruncular areas,respectively.Functional analysis showed that the DEPs were mainly associated with immune response,nutrient transport and utilization,as well as proteasome-mediated proteolysis.Conclusion:These analysis imply that dysfunction of these biological processes or pathways of DEP in the endometrium is highly associated with early pregnancy loss.In addition,many proteins that are essential for the establishment of pregnancy showed dysregulation in the endometrium of non-pregnant ewes.These proteins,as potential candidates,may contribute to early pregnancy loss.
本文主要介绍了两项新发展的繁殖技术——母猪定时输精技术和精子微胶囊技术。定时输精技术是以G n RH为基础,结合P MSG、HCG、FSH、LH等激素的作用,使母猪卵泡发育和排卵同步化,进而实施定时输精;精子微胶囊技术则是在保持精子正常生理代谢和受精能力的同时,应用高分子聚合材料将原精液进行包裹,并通过常规的输精方式对母猪实施单次输精的一种技术。本文分别对此两项技术的研究背景、技术原理、生产上的应用及其前景等方面进行了概述,为相关研究人员和养猪企业提供参考。
