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国家自然科学基金(30700356)

作品数:4 被引量:22H指数:3
相关作者:姜颖刘伟虞朝辉叶桦厉有名更多>>
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发文基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
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乙肝纤维化分级相关血浆蛋白标志物的定量蛋白质组筛选被引量:5
2011年
非创伤性肝纤维化检测对于评价肝炎进程、临床治疗以及药效评价等方面,都具有非常重要的作用.通过N末端标签的蛋白质组定量手段,从血浆中筛选肝纤维化分级相关的蛋白候选标志物.首先利用目前纤维化诊断黄金标准——肝穿病理检测,获得纤维化不同分级的合格样本血浆,并对肝纤维化各级间的血浆混合样本进行了蛋白质组定量比较分析,发现了72个与乙肝纤维化分级呈明显相关性的血浆蛋白,构成了一个有重要参考价值的、纤维化分级诊断的候选蛋白标志物库.用WB法进一步验证了定量结果的准确性.利用IPA在线分析后获得了4个生理代谢网络图,提示这些差异蛋白在肝纤维化进程中代表的不同功能影响.总之,利用N末端标签定量蛋白质组技术,全面定量挖掘了血浆中与乙肝纤维化分级相关的差异蛋白及变化规律,对于加深肝纤维化发病机制的认识和理解具有很大帮助,同时也为肝脏纤维化临床诊断和治疗提供有益信息和参照.
李树龙刘新魏来王慧芬张继阳魏汉东钱小红姜颖贺福初
关键词:定量蛋白质组学肝脏纤维化标志物血浆乙型肝炎
An integrated view of the correlations between genomic and phenomic variables被引量:2
2009年
Genome sequencing opened the flood gate of "-omics" studies,among which the research about correlations between genomic and phenomic variables is an important part. With the development of functional genomics and systems biology,genome-wide investigation of the correlations between many genomic and phenomic variables became possible. In this review,five genomic variables,such as evolution rate(or "age" of the gene) ,the length of intron and ORF(protein length) in one gene,the biases of amino acid composition and codon usage,along with the phenomic variables related to expression patterns(level and breadth) are focused on. In most cases,genes with higher mRNA/protein expression level tend to evolve slowly,have less intronic DNA,code for smaller proteins,and have higher biases of amino acid composition and codon usage. In addition,broadly expressed proteins evolve more slowly and are shorter than tissue-specific proteins. Studies in this field are helpful for deeper understanding the signatures of selection mediated by the features of gene expression and are of great significance to enrich the evolution theory.
Dong Yang,Ying Jiang,Fuchu He State Key Laboratory of Proteomics,Beijing Proteome Research Center,Beijing Institute of Radiation Medicine,Beijing 102206,China
关键词:密码子使用编码蛋白质功能基因组学进化速率
Plasma biomarker screening for liver fibrosis with the N-terminal isotope tagging strategy被引量:6
2011年
A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.
LI ShuLongLIU XinWEI LaiWANG HuiFenZHANG JiYangWEI HanDongQIAN XiaoHongJIANG YingHE FuChu
关键词:同位素标记血浆蛋白定量蛋白质组学
非酒精性脂肪性肝病蛋白质组学研究进展被引量:9
2011年
非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)是一种常见慢性肝脏疾病,其发病率呈逐年上升趋势,但发病机制尚未明确,诊疗手段仍不完善.蛋白质组学(proteomics)的出现使NAFLD研究有了进一步的发展,相关研究已达21个.目前,蛋白质组学技术可以研究疾病相关的分子改变,从而寻找新的生物标志物和治疗靶标.在此,对蛋白质组学在NAFLD诊断及分期、发病机制和其他相关领域研究进展作一个较为全面的综述.首先,对研究中遇到的研究对象、样本种类、实验方法和标志物特征选择进行经验性总结.其次,除了介绍如何运用蛋白质组学研究病因、危险因素和重要分子在NAFLD发病机制中的作用,还介绍NAFLD发病机制的亚细胞蛋白质组学、修饰蛋白质组学以及蛋白质组学与转录组学相结合的研究实例.此外,对差异蛋白质的分析策略和价值作了重点阐述,收集到一些有望成为NAFLD治疗靶标的候选分子.最后,结合新技术展望研究新空间,以期能够有助于推动蛋白质组学在寻找新的疾病标志物、探索疾病分子机制和治疗靶标中开辟新的途径.
叶桦刘伟虞朝辉厉有名姜颖
关键词:蛋白质组学非酒精性脂肪性肝病生物标志物发病机制
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