The capability of our newly developed DNA-machine-driven strategy in tuning the association rate of DNA- AuNPs was compared with that of linker-addition strategy which has potential practical applications in different fields. The new established strategy shows its superiority to the linker-addition strategy in tuning the association rate of DNA-AuNPs in both pre-incubation treatment and non-incubation treatment conditions since its two components （a complex and a catalyst- oligonucleotide） can be individually optimized to make the machine run at an optimal rate. This strategy will provide a more convenient and flexible option in designing an oligonucleotide detection system and building a complex and versatile device.
