Objective To evaluate the efficacy of rabbit lens capsular bag by using 5-fluorouracil(5-FU) in combination with mitomycin C (MMC) in preventing posterior capsule opacification (PCO) and its mechanism. Methods Forty New Zealand rabbits (80 eyes) received continuous curvilinear capsulorhexis (CCC,its diameter approximately 3 millimete) and phacoemulsification,and were randomly divided into 2 groups:The right eyes of the rabbits lens capsular bags of the experimental group (twenty New Zealand rabbits)were in jected with methylcellulose (MC) contained 12.5 mg/ml 5-FU and 0.2mg/ml MMC,and the left eyes of that were injected with only MC as vacuity control group;While the right eyes of the rabbits lens capsular bags of the control group (twenty New Zealand rabbits) were injected with methylcellulose (MC) contained 0.2mg/ml MMC,and the left eyes of that were in jected with MC contained 12.5 mg/ml 5-FU.The anterior chamber of all subjects were filled with sodium hyaluronate (HA).Five minuts late,the anterior chambers and lens capsular bags of all subjects were permutated by irrigating solution.Some of the subjects were implantated intraocular lens (IOL) in lens capsular bags.In the two-month to twenty-month follow-up after the operation,all eyes were examined by slit-lamp microscopy,the PCO were observed and compared between the two groups;the histopathologic examination of the lens capsule membran and IOL,the wholemount technique of the lens capsule membran,the electron microscope inspection of the IOL were also observed in different period. Results For clinical study,the PCO appeared in the vacuity control group two weeks after the operation.Some of the psuedophakic subject were formed frontal membrane of the IOL.The longer follow-up after the operation,the stronger PCO were taken place.The PCO did not appeare any more in the experimental group twenty-month follow-up after the operation,while the control group appeare somewhat of a PCO.The histopathologic examination showed that the proliferation of lens epithelial ce
