[Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. [Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. [Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. [Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities.
被子植物的胚珠微小,手工分离胚珠内的珠心比较困难（胡适宜,1994）。目前,人们多是借助于纤维素酶和果胶酶等的酶解技术,完成珠心、胚囊、卵细胞和合子等微小结构的人工分离（周嫦等,1982；Huang et al．，1990；Kovacs et al．，1994；Tian et al．，1997；杨弘远等，1998；2001；Uchiumi et al．，2006）。由于胚囊和合子经酶处理后，
