Objective:To investigate the effects of Toll-like receptor 4 antagonist Eritrane on neurogenesis and gamma-aminobutyric acid glutamate balance in the prefrontal lobe and hippocampus of depressive rats.Methods:100 healthy SD rats were divided into healthy control group, blank control group and Eritram group (low dose group, medium dose group and high dose group), 20 rats in each group. The depression model of rats in blank control group and Salvia miltiorrhiza group was established. After the completion of the model, rats in the blank control group were injected with normal saline and rats in the Eritrean group were injected with high, medium and low doses of Eritrean injection respectively. After 21 days of continuous administration, the total distance of spontaneous activity, the immobility time of forced swimming, the levels of gamma-aminobutyric glutamate (GABA), glutamate (Glu) and Toll-like receptor 4 (TLR-4) protein in prefrontal lobe and hippocampal neurons were measured and compared. Pearson correlation test was used to analyze the correlation between TLR4 and GABA, Glu levels in depressive rats.Results: Compared with the healthy control group, the total spontaneous activity distance of the blank control group and Eritrean group decreased significantly, and the immobility time of forced swimming increased significantly (P<0.05). There was a significant difference between the blank control group and Eritrean group (P<0.05). The rats in Eritrean group spontaneously survived with the increase of dose. The total distance increased, while the immobility time of forced swimming decreased (P<0.05). Compared with the healthy control group, the levels of GABA in the prefrontal lobe and hippocampus of rats in blank control group and Eritrean group decreased significantly, the levels of Glu and TLR4 increased significantly, and the levels of GABA in Eritrean group were higher than those in blank control group, and the levels of Glu and TLR4 were lower than those in blank control group. TLR4 and TLR4 decreased signif
目的:探讨重复经颅磁刺激(r TMS)对抑郁症患者的疗效及对患者外周血PDLIM5基因转录水平的影响。方法:正常健康者24例纳入正常组,抑郁症患者27例纳入抑郁组。抑郁组患者接受r TMS治疗2周。于治疗前、后,行汉密尔顿抑郁量表(HAMD)评分,并检测外周血中PDLIM5 m RNA转录水平。结果:抑郁组HAMD总分明显高于对照组(P<0.05)。r TMS治疗后,抑郁组"有效"13例(48.1%),"明显缓解"8例(29.6%);HAMD总分较治疗前明显下降(P<0.01),但仍高于对照组(P<0.05)。治疗前抑郁组外周血PDLIM5 m RNA转录水平明显低于对照组(P<0.05),治疗后抑郁者组外周血PDLIM5 m RNA表达水平与治疗前差异无统计学意义(P>0.05)。治疗前后,抑郁组HAMD总分与外周血PDLIM5 m RNA转录水平无明显相关性(P>0.05)。结论:r TMS治疗抑郁症有效,对外周血PDLIM5转录水平无影响。
