The inhibitory effect of wortmannin on leukemic cells and the possible mechanisms were examined.K562 cells were treated with wortmannin of various concentrations(3.125-100 nmol/L) for 0-72 h.MTT assay was used to evaluate the inhibitory effect of wortmannin on the growth of K562 cells.Cell apoptosis was detected by both Annexin-ⅤFITC/PI double-labeled cytometry and transmission electron microscopy(TEM).The expression of p-Akt,T-p-Akt,NF-κBp65 and IKK-κB was determined by Western blotting and reverse transcription-polymerase chain reaction(RT-PCR). Our results showed that wortmannin obviously inhibited growth and induced apoptosis of K562 cells in vitro in a time-and dose-dependent manner.The IC50 value of wortmannin for 24 h was 25±0.14 nmol/L.Moreover,wortmannin induced K562 cells apoptosis in a dose-dependent manner.TEM revealed typical morphological changes of apoptosis in wortmannin-treated K562 cells,such as chromatin condensation,karyopyknosis,karyorhexis and apoptotic bodies.Additionally,several important intracellular protein kinases such as p-Akt,NF-κBp65 and IKK-κB experienced degradation of various degrees in a dose-dependent manner both at protein level and transcription level when cultured with wortmannin,but the expression of total Akt showed no change.It is concluded that wortmannin can inhibit the proliferation and induce apoptosis of K562 leukemia cells possibly by down-regulating the survival signaling pathways(PI3K/Akt and NF-κB channels).
The roles of voltage-dependent K+ channels during activation and damage in alveolar macrophages (AMs) exposed to different silica particles were examined. Rat AMs were collected by means of bronchoalveolar lavage, and were adjusted to 5×105/mL. After AMs were exposed to different concentrations (0, 25, 50, 100, 200 μg/mL) of quartz particles and 100 μg/mL amorphous silica particles for 24 h, the voltage-depended K+ current in AMs was measured by using patch clamp tecnique. Meanwhile the leakage of lactate dehydrogenase (LDH) and the viability of AMs were detected respectively. Patch clamp studies demonstrated that AMs possessed outward delayed and inward rectifying K+ current. Exposure to quartz particles increased the outward delayed K+ current but it had no effect on inward rectifier K+ current in AMs. Neither of the two K+ channels in AMs was affected by amorphous silica particles. Cytotoxicity test showed that both silica particles could damage AM membrane and result in significant leakage of LDH (P<0.05). MTT studies, however, showed that only quartz particles reduced viability of AMs (P<0.05). It is concluded that quartz particles can activate the outward delayed K+ channel in AMs, which may act as an activating signal in AMs to initiate an inflammatory response during damage and necrosis in AMs induced by exposure to quartz particle. K+ channels do not contribute to the membrane damage of AMs.
