Thirty-six strains of endophytic Colletotrichum species were isolated from leaves of Bletilla ochracea Schltr.(Orchidaceae)collected from 5 sites in Guizhou,China.Seventeen different species,including 7 new species(namely C.bletillum,C.caudasporum,C.duyunensis,C.endophytum,C.excelsum-altitudum and C.guizhouensis and C.ochracea),8 previously described species(C.boninense,C.cereale,C.destructivum,C.karstii,C.liriopes,C.miscanthi,C.parsonsiae and C.tofieldiae)and 2 sterile mycelia were identified.All of the taxa were identified based on morphology and phylogeny inferred from multi-locus sequences,including the nuclear ribosomal internal transcribed spacer(ITS)region,partial genes ofβ-tubulin(TUB2),actin(ACT)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH).Comprehensive morphological descriptions and illustrations are provided for new species.Our investigation indicates a high diversity of Colletotrichum species in B.ochracea.
