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国家自然科学基金(30671580)

作品数:4 被引量:3H指数:1
相关作者:李建华张西臣杨桂连宫鹏涛赵权更多>>
相关机构:吉林大学吉林农业大学更多>>
发文基金:国家自然科学基金国家高技术研究发展计划更多>>
相关领域:农业科学生物学更多>>

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柔嫩艾美耳球虫端粒DNA和TERT基因克隆及端粒酶活性测定
本研究以E.tenella为研究对象,应用单卵囊分离技术成功分离具有较强感染力地方分离株。在此基础上,成功构建了富含端粒序列文库,进一步通过Southern杂交和酶切筛选阳性克隆,确定了E.tenella端粒DNA重复序...
杨桂连李建华张西臣宫鹏涛杨举赵全
关键词:柔嫩艾美耳球虫TERT端粒
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微小隐孢子虫端粒序列及端粒酶活性的研究
2012年
为了探讨我国微小隐孢子虫(C.parvum)的端粒序列,试验根据国外已报道的端粒重复序列5'-CCTAAA-3'设计寡聚核苷酸探针(CCTAAA)5,并用地高辛标记,C.parvum基因组DNA经Bal31-HindⅢ酶切后进行Southern印迹杂交鉴定;并在此基础上,采用改进的端粒重复序列扩增(TRAP)法首次对子孢子和卵囊时期C.parvum的端粒酶活性进行检测。结果表明:C.parvum基因组DNA与探针(CCTAAA)5杂交,获得了较好的杂交条带,随着Bal31-HindⅢ酶切时间的延长,杂交信号逐渐减弱,说明C.parvum端粒序列定位在染色体的末端,与国外报道的C.parvum端粒序列一致,为5'-CCTAAA-3';检测C.parvum端粒酶活性时发现,子孢子时期端粒酶具有活性,卵囊中未检测到端粒酶活性。
赵娜刘慧李建华宫鹏涛杨举李赫张西臣
关键词:微小隐孢子虫端粒端粒酶
E.tenella吉林株单卵囊的分离及PCR鉴定(英文)
2009年
[Objective] In order to get a purified strain to carry out the relative molecular biology research about E.tenella. [Method] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E.tenella. [Conclusion] The inoculation of single oocyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty, increased infection rate and provided good materials for biological research of coccidian.
杨桂连李建华张西臣赵权宫鹏涛蔡亚南张国才
关键词:E.TENELLAPCR
Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens被引量:2
2009年
Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry.Enhanced green fluorescent protein(EGFP) tagged recombinant Bacille Calmette-Guerin(rBCG),as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study.Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response.The colonization of rBCG in liver,spleen,lung,kidney and caecum was observed by laser confocal microscopy.Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization.These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
WANG QiuYue1,LI JianHua1,ZHANG XiChen1,LIU ChengWu2,CAO LiLi1,REN KeYan3,GONG PengTao1,CAI YaNan11 College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China
关键词:COCCIDIOSISEGFPRHOMBOIDCONFOCAL
寄生虫端粒及端粒酶的研究进展被引量:1
2009年
端粒(Telomere)是真核生物染色体末端由重复DNA序列和蛋白质结合形成的复合结构,能够稳定和维持染色体的完整性,在DNA的复制过程中防止染色体末端融合及有丝分裂时染色体分离方面起着重要作用。端粒酶(Telomerase)是真核生物染色体末端具有逆转录酶特性的核蛋白,可以利用自身携带的RNA模板合成染色体末端DNA,并添加到染色体末端以维持端粒长度的稳定,其活性与细胞的分裂和死亡、细胞癌变以及细胞衰老等有关。近年来,有关端粒及端粒酶的研究异常活跃,
杨桂连李建华张西臣
关键词:端粒酶重复DNA序列寄生虫染色体分离真核生物蛋白质结合
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