Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou,Zhejiang province of China.The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test,electron microscope observation,hypersensitive reaction,Koch’s postulates,physiological and biochemical test,biolog,fatty acid methyl esters analysis (FAMEs),enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR),16s rRNA sequences analysis,and comparative analysis with 7 type strains and 3 reference strains.This is the first report on mulberry disease caused by Enterobacter spp.in the world providing new evidence on induction of the plant disease in this genus.The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.
Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It's quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease.The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency,and to develop an accurate,rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease.The results showed that all the tested strains of B.glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods,while others showed negative PCR result.The bacteria could be detected at the concentrations of 1×104CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method.B.glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1:100.
