A new method that nanometer-liposomes could penetrate the cell membrane to deliver their encapsulated material into cells was investigated.Nanometer sized-liposomes were produced from phosphatidylcholine to encapsulate fluorescent dyes and FITC labeled superoxide dismutase(SOD-FITC) by an ultrasonic method.The size distribution of liposomes was determined with a Laser Particle Analyser.The fluorescence images demonstrate that liposomes can transfer fluorescent dyes(FITC,Rhodamine B),which are not cell membrane permeable,into cells.Single cell analysis of the delivered SOD-FITC with microchip electrophoresis revealed that the density of liposomes and interaction-time between liposomes and cells affected the efficiency of intracellular delivery of encapsulated material.
In this paper the design and development of a novel experiment system and method for organic synthesis in a microfluidic were described chip.The phase-transfer reaction for the synthesis of 4-methoxy-benzaldehyde oxime from 4-methoxybenzaldehyde and hydroxylammonium chloride was carried out in glass microfluidic chips by using a negative pressure system to control the transformation reactants through the microchannels at a constant flow rate.The effect of reaction time on the yield was determined and compared with the standard batch system.The demonstrated advantages of organic synthesis in microfluidic chip included faster reaction rate,less consumption of reactants and labor contaminant,which proved microfluidic chip to be a powerful tool for synthetic applications.
