Objective:To observe the anti-virus effects of andrographolide(AD) on the retinoic acid-inducible gene-l(RIG-l)-like receptors(RLRs) signaling pathway when immunological cells were infected with H1N1.Methods:Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation,and immunological cells were harvested after cytokines stimulation.Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line(16HBE).The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium(MTT) assay.After the virus infected cell model was established,AD was added into the medium as a treatment intervention.After 24-h co-culture,cell supernatant was collected for interferon gamma(IFN-γ) and inteiieukin-4(IL-4) enzyme-linked immunosorbent assay(ELISA) detection while immunological cells for real-time polymerase chain reaction(RT-PCR).Results:The optimal concentration of AD for anti-virus effect was 250 μg/mL IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus,RIG-I,IFN-β promoter stimulator-1(IPS-1),interferon regulatory factor(IRF)-γ,IRF-3 and nuclear transcription factor κB(NF-κB) mRNA levels increased significantly(P<0.05).When AD was added into co-culture medium,the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased,RIG-I,IPS-1,IRF-γ,IRF-3 and NFk B decreased significantly in each group with significant statistic differences(P<0.05).Conclusions:The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality.And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.
OBJECTIVE:To observe the effect of the Traditional Chinese Medicine,modified Taohongsiwu decoction,versus pyridoxine on patients with hand-foot syndrome(HFS)from capecitabine,sorafenib,and gefitinib chemotherapy for gastric,lung,breast,colon,or rectal cancer.Also,to compare quality of life of patients in each group.METHODS:Patients were assigned randomly to group A or B.Group A was given modifiedTaohongsiwu decoction to soak hands and feet for 30 min,once daily.Group B was given 100 mg pyridoxine orally,twice daily.After a 2-week treatment,thetherapeutic effect was assessed by observing three major symptoms,including pain,ulceration,and muscular atrophy.This was assessed with the HFS-14 questionnaire.RESULTS:Significant differences were observed between the two groups in pain relief,and improvement of daily life,walking,and interpersonal communication(P<0.01).No significant differences in driving ability or interpersonal relationships were found.After 2 weeks,the effective rate was 88.3%in group A,which was significantly higher than the50%in group B(P=0.00).CONCLUSION:Modified Taohongsiwu decoction is effective in the treatment of patients with HFS.It improves patients'quality of life according to the HFS-14.
目的:观察比较新加香薷饮对湿热环境下流感病毒性肺炎小鼠的治疗作用。方法:42只C57BL/6J小鼠随机分成6组,正常环境组每天正常饲养,湿热环境各组人工气候箱湿热环境中饲养7天。需要病毒攻击的各组,使用FM1病毒滴鼻。药物组灌胃治疗,利巴韦林用量为0.07 g/(kg·d),新加香薷饮用量为23 m L/(kg·d)。通过小鼠体重变化、肺部组织HE染色、流式细胞术等实验,测定与比较新加香薷饮对湿热环境下流感病毒性肺炎小鼠治疗作用效果与机制。结果:湿热环境一定程度上能促进病毒感染后的炎症反应,差异有统计学意义(P<0.05)。利巴韦林和新加香薷饮在病毒感染后具有抗炎效果,差异有统计学意义(P<0.05)。结论:湿热环境对于流感病毒性肺炎具有促炎作用。新加香薷饮对湿热环境下流感病毒性肺炎小鼠具有抗病毒作用,且效果优于利巴韦林。
