Objective:To investigate the effect of Ermiao Recipe(二妙方,EMR)with medicinal guide Angelicae Pubescentis Radix(APR)on the homing of bone marrow stem cells(BMSCs)to focal zone in osteoarthritis(OA)rats.Methods:Forty-eight Sprague-Dawley rats were randomly assigned to the sham-operated,model,EMR,and EMR plus APR groups(12 rats in each group).The OA rat model was induced by anterior cruciate ligament transection and medial meniscus resection.All rats were injected with recombinant human granulocyte colonystimulating factor[rhG-CSF,30μg/(kg-d)for continuous 7 days],and rats in the EMR and EMR plus APR groups were treated with EMR or EMR plus APR at 1.6 or 1.9 g/(kg·d)for 3 or 6 weeks,respectively.Cartilage histopathologic changes were observed by hematoxylin and eosin staining.Chondrocytes apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining,lnterleukin-1 B(IL-1β),tumor necrosis factorα(TNF-α),bone morphogenetic protein 2(BMP-2),and transforming growth factor beta-1(TGF-β_1)in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay assay.Matrix metalloproteinase(MMP)-13,tissue inhibitors of metalloproteinase(TIMP)-1,bromodeoxyuridine(BrdU),cluster of differentiation 34(CD34),and stromal cell derived factor 1(SDF-1)were measured by immunohistochemistry assay.Results:EMR and EMR plus APR significantly inhibited articular cartilage damage and synovium inflammation in OA rats at 3 or 6 weeks of treatment,the most obvious changes in these parameters were found in the EMR plus APR group.At 6 weeks,compared with EMR treatment,EMR plus APR remarkably inhibited chondrocytes apoptosis and the release of IL-1βand TNF-α,obviously decreased MMP-13 expression,and significantly increased expressions of proteoglycan,collagen,typeⅡcollagen and TIMP-1,serum levels of BMP-2 and TGF-β_1 as well as expressions of BrdU,CD34 and SDF-1 in cartilage articular(P<0.01 or P<0.05).Conclusion:The medicinal gu
OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.
Ying XuGuojing DaiQian LiuHongwei ZhuWeiheng ChenPing ZhangTiejun ZhaoNa Lin
