Fungi Z4 and Z8, isolated from the heavy metal polluted soil, have strong resistance to Cd and Pb. The strains were identified on the base of their morphology and internal transcribed spacers(ITS) region. Pot experiments were conducted to study the effect of two strains(Z4 and Z8) on the growth and accumulation of Cd and Pb of Guizhou oilseed rape. The results show that strains Z4 and Z8 belong to Mucor circinelloides and Mucor racemosus, respectively. The heights of Guizhou oilseed rape inoculated with strain Z8 increase by 47.90% than the control. The highest fresh mass is found in the plant with Z4/Z8, which is enhanced by160.81%. Pot experiments show that Z4/Z8 inoculums can accelerate accumulation of heavy metals in the plant. The contents of Cd and Pb are increased by 117.60% and 63.48%, respectively. Meanwhile, the heavy metal concentrations in potting soil with the two strains are found to be lower than those of the control, and the concentrations of Cd and Pb are decreased by 60.57% and 27.12%,respectively.
目的:研究汉族妇女中MDM2基因SNP309多态性与宫颈癌易感性及临床病理学参数之间的关系。方法:用DNA抽提试剂盒从研究对象的外周血标本中抽提基因组DNA,其中宫颈癌患者105例,正常对照组140例;用聚合酶链式反应-限制性片段长度多态(PCR-RFLP)和直接测序方法测定MDM2-SNP309单核苷酸多态基因型。结果:宫颈癌患者的MDM-SNP309 G等位基因频率显著高于对照组(60.0% vs 48.6%,P=0.012;OR=1.59,95% CI=1.11～2.28);宫颈癌与对照组之间的GG、TG和TT等位基因型的分布差异有统计学意义,其中GG等位基因型在宫颈细胞癌中的频率显著高于对照组(36.2% vs 18.6%,P=0.016;OR=2.58,95% CI=1.19～5.61)。在宫颈癌组中,淋巴转移阳性组MDM2-SNP309 GG等位基因型显著高于淋巴转移阴性组(31.8%vs11.5%,P<0.05),SNP309单核苷酸多态性分布与肿瘤组织学类型、病理分级及肿瘤大小无关。结论:MDM2基因SNP309 GG基因型是宫颈癌发生的遗传易感因素,与宫颈癌的淋巴转移发生有相关性。
In this paper, we report a high-performance P3HT/PCBM bulk-heterojunction solar cell with a power conversion efficiency of 4.85% fabricated by adjusting the polymer crystallinity and nanoscale phase separation using an ultrasonic irradiation mixing approach for the polymer. The grazing incidence X-ray diffraction, UV/Vis spectroscopic, and atomic force microscopic measurement results for the P3HT/PCBM blend films reveal that the P3HT/PCBM film fabricated by ultrasonic irradiation mixing of the P3HT and PCBM solutions for 10 min has a higher degree of crystallinity, a higher absorption efficiency, and better phase separation, which together account for the higher charge transport properties and photovoltaic cell performance.
