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国家自然科学基金(30371028)

作品数:3 被引量:8H指数:1
相关作者:蒋思文孙智达刘丽娜梁秋菊彭健更多>>
相关机构:华中农业大学更多>>
发文基金:国家自然科学基金湖北省科技攻关计划更多>>
相关领域:农业科学更多>>

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猪血液和肌肉组织DNA甲基化含量的测定被引量:7
2007年
【目的】测定纯种大白猪(LW)、长白猪(L)、梅山猪(M)及其LW×L、L×LW、LW×M和M×LW等杂种猪血液和肌肉组织DNA甲基化含量,比较亲本与杂种DNA甲基化含量的差异,为揭示杂种优势的分子机制提供依据。【方法】采用高效液相色谱法测定血液和肌肉组织中甲基化含量。【结果】163份肌肉组织DNA样平均甲基化含量为16.92%,182份血液样平均甲基化含量为6.49%,两者之间差异达到了极显著水平(P<0.01),血液中DNA甲基化含量均低于10%,L×LW杂交猪最高,达到了9.93%,而长白猪最低,仅为2.97%;大白猪和长白猪之间差异极显著(P<0.01),而大白猪和梅山猪之间差异不显著(P>0.05);LW×L与L×LW杂种猪,LW×M与M×LW杂种猪之间均存在显著性差异(P<0.05)。肌肉组织中DNA甲基化含量3个纯种亲本之间无显著性差异(P>0.05);LW×L与L×LW杂种猪、LW×M与M×LW杂种猪之间差异均不显著,但是两个不同杂交组合,即LW×L、L×LW与LW×M、M×LW杂种猪之间差异显著(P<0.05)。【结论】本研究建立了测定猪血液和肌肉组织中DNA甲基化含量的高效液相色谱测定方法;DNA甲基化含量在血液和肌肉组织中差异显著,且具有组织特异性。
梁秋菊刘丽娜彭健孙智达蒋思文
关键词:高效液相色谱DNA甲基化
Determination and Difference Analysis of DNA Methylation Content Both in Blood and Muscle Tissue of Pigs
2008年
In this experiment, DNA samples from parental lines Large White, Landrace, and Meishan pigs, and their hybrids Large White × Landrace, Landrace × Large White, Large White × Meishan, and Meishan × Large White pigs were used for the determination of DNA methylation content in both blood and muscle tissue. The differences about DNA methylation content between parental lines and their hybrids were analyzed. These will offer theoretical support from molecular level for heterosis. High performance liquid chromatography (HPLC) was firstly used to detect DNA methylation content. The average DNA methylation content in 163 DNA samples of muscle tissue was 16.92%, whereas, the average DNA methylation content in 182 samples of blood was 6.49%, the difference between which was especially prominent (P 〈 0.01). In blood, the methylation content was lower than 10%, with the highest level of 9.93% in Landrace x Large White and the lowest of 2.97% in Landrace. The difference between Large White and Landrace was highly significant (P 〈 0.01), but not significant between Large White and Meishan; and the differences between reciprocal cross hybrids in both hybrid systems were significant (P 〈 0.05). In muscle tissue, the differences in methylation content among three parents were not significant (P 〉0.05); and the differences between reciprocal cross hybrids in both hybrid systems were not significant (P 〉 0.05), but between different hybrid systems, the hybrids had a significant difference (P 〈0.05). The average methylation content in muscle samples was higher than that in blood samples, and the methylation in different tissues was different.
LIANG Qiu-juLIU Li-naPENG JianSUN Zhi-daJIANG Si-wen
关键词:HPLCPIG
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