In this study, a recombinant Pichia pastoris expression system was developed to express HPV16 L1 protein that was driven by a strong AOX1 promoter. HPV16L1 gene was cloned into vector pPICZ%α%B. HPV16 L1 protein expression induced by methanol was screened by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blotting. The results indicate that the HPV16 L1 protein is secreted by the recombinant P. pastoris, and the purified HPV16 L1 protein can self-assemble into virus-like particles(VLPs), which show a good immunogenicity and induces high-titer antibody in mice.
LIU Da-wei ZHANG Yu YU Xiang-hui JIANG Chun-lai CHEN Yue WU Yong-ge JIN Ying-hua NIU Jun Qu Ning LIU Ming KONG Wei
