测定不同含水量、冷解冻方式处理的苦槠离体胚内源SOD和α-淀粉酶活性,研究其超低温保存效果.结果表明:不同处理苦槠离体胚的SOD和α-淀粉酶活性差异显著,含水量水平是影响其液氮保存的关键因素,而冷解冻方式也是一重要因素.12%含水量和缓冻快解法处理,苦槠离体胚的超低温保存效果最好,SOD和α-淀粉酶活性最高,分别达212.77 U/h和4.764 mg/m in.
The quality character, the dehydrogenase activities and the α amylase activity of Castanea mollissima conserved materials, including the seeds and the excised embryos, were analyzed for purpose of the feasibility of long term storage associated with cryopreservation. The results showed that moisture content was the critical factor deciding the cryopreservation effects of seeds, which were desiccated down to 20% necessarily. Under the conditions of the cryoprotectants, the freezing injury reduced largely, and dehydrogenase activities remained well. Moreover the cryoconserved scale of moisture content was enlarged. A proper combination of cryopreserved factors contributed to maintain the seed vigor, the dehydrogenase activity and α amylase activity of excised embryos during cryopreservation.
