国家自然科学基金(21275027)
- 作品数：1 被引量：7H指数：1
- 相关作者：郑旭王晓峰王建华陈旭伟更多>>
- 相关机构：东北大学更多>>
- 发文基金：辽宁省自然科学基金国家自然科学基金更多>>
- 相关领域：理学更多>>
- Nickel Nanoparticle Decorated Graphene for Highly Selective Isolation of Polyhistidine-tagged Proteins
- ＜正＞Graphene has attracted great attentions due to its unique, outsting physical, chemical properties since 200...
- Jiawei LIUTing YANGLinyu MAXuwei CHENJianhua WANG
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- Carbon Dots-induced Growth of Silver Nanoparticles, Applications in Cysteine Detection
- ＜正＞Carbon dots are both excellent electron donors, excellent electron acceptors. In present study, we report t...
- Liming SHENMeiling CHENXuwei CHENJianhua WANG
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- 超高容量氧化石墨烯/碳纳米管气凝胶用于血红蛋白的吸附分离被引量：7
- 2015年
- 采用冷冻干燥技术制备了三维氧化石墨烯/碳纳米管(GO/CNTs)气凝胶,在疏水作用下,该气凝胶对蛋白质表现出良好的吸附性能.在p H=8.0时,GO/CNTs气凝胶对血红蛋白的吸附效率很高,而对白蛋白基本不吸附,从而可实现血红蛋白的选择性吸附分离.在最佳吸附条件下,GO/CNTs气凝胶对血红蛋白的吸附容量高达3793.3 mg/g,是去除高丰度血红蛋白的高效材料.吸附后的血红蛋白可以使用p H=11.5的BrittonRobisin缓冲液有效洗脱,回收率为67%.圆二色光谱结果表明,GO/CNTs气凝胶具有良好的生物相容性,其表面发生的吸附/洗脱过程对血红蛋白的构象基本无影响.将GO/CNTs气凝胶用于人全血样品中血红蛋白的选择性分离,SDS-聚丙烯酰胺凝胶电泳结果表明得到了较高纯度的血红蛋白.
- 陈旭伟郑旭王晓峰王建华
- 关键词：氧化石墨烯碳纳米管气凝胶血红蛋白
- Multi-Protein Discrimination by Ionic Liquid-Modified QDs Fluorescent Sensor
- ＜正＞Multidimensional sensing devices have received increasing attention because they are able to read out the t...
- Ling WEIShuai CHENXu-Wei CHENJian-Hua WANG
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- In situ Growth of β-FeOOH Nanorods on Graphene Oxide with Ultra-high Relaxivity for in vivo Magnetic Resonance Imaging and Cancer Therapy
- ＜正＞β-FeOOH has shown promise as a new contrast agent in magnetic resonance imaging （MRI）. Its special morpholo...
- Hui WANGMeiling CHENMingli CHENJianhua WANG
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- A FRET Ratiometric Fluorescence Sensing System for Mercury Detection and Intracellular Colorimetric Imaging in Live Hela Cells
- ＜正＞Mercury is one of the most toxic heavy metals for its bio-accumulative and persistent character with the da...
- Bo HULinlin HUMingli CHENXuwei CHENJianhua WANG
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