Objective:The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of r BMSCs.Methods:r BMSCs were harvested from SD rats,and determination of alkaline phosphatase(ALP)activity,quantification of mineralization by Alizarin Red S staining,and the m RNA expression of osteogenic differentiation markers(Runx2,BMP-2,and OSX)by RT-PCR after r BMSCs stimulated by osteogenic induction with(0.1–10)μg/m L of quercitrin,quantification of Lipid droplet by Oil Red O staining and the m RNA expression of adipogenic differentiation marker(,and a P2)by RT-PCR after r BMSCs stimulated by adipogenic induction with(0.1-10)μg/m L of quercitrin.Results:Quercitrin can up-regulate the m RNA expression of osteogenic differentiation markers(Runx2,BMP-2,and OSX)and increase ALP activity and mineralization after osteogenic induction,on the other hand quercitrin can suppress the m RNA expression of adipogenic differentiation markers(,and a P2)and decrease lipid droplet after adipogenic induction.Conclusion:This study suggested that quercitrin not only stimulated osteogenic differentiation but also inhibited adipogenic differentiation of r BMSCs,which was associated with the up-regulation of Runx2,BMP-2,and OSX m RNA expression and the down-regulation of,and a P2 m RNA expression.
Zi-yi GuanLan-ying ChenXue-liang LiYa-ru CuiRong-hua Liu
