目的探讨兔膝骨关节炎发展进程中软骨下骨血管生成的时间依从性的变化,研究软骨下骨血管新生在OA发病机制中的作用。方法 30只新西兰大白兔随机分为前交叉韧带切除组(ACLT组)、对照组,每组15只。ACLT组右膝前交叉韧带切除造模,对照组不作任何处理。分别于术后4周、8周、12周取材。取兔膝关节标本进行大体观察,切片行组织学番红O染色及OARSI病理评分,评价软骨退变。行免疫组化VEGF染色,观察软骨下骨中血管生成情况,同时用Image Pro Plus6.0图像分析软件进行骨软骨交界血管数量密度的计数检测。结果 ACLT组术后4周即可见软骨退变,8周和12周时退变进一步加重,番红O染色基质丢失、软骨变薄及裂隙生成、软骨下骨裸露。对照组无明显软骨退变,不同组关节软骨OARSI评分有统计学差异(F=440.828,P<0.01)。切片免疫组化血管内皮生长因子(VEGF)染色示ACLT组阳性表达,ACLT组的血管入侵软骨数目明显高于对照组,且OA各时间点之间有统计学差异(F=72.733,P<0.01)。结论软骨下骨的血管生成是OA的早期病变,OA中骨-软骨复合单元的血管增生与软骨退变相关。血管侵入软骨的数量密度与OA发展呈时间依从性增加变化。
Most related investigations focused on the effects of borate glass on cell proliferation/biocompatibility in vitro or bone repair in vivo;however,very few researches were carried out on other cell behaviors.Three novel borate bioglasses were designed as scaffolds for bone regeneration in this wok.Comparative effects of three bioglasses on the behaviors of osteoblastic MC3T3-E1 cells were evaluated.Excellent cytocompatibility of these novel borate bioglasses were approved in this work.Meanwhile,the promotion on cell proliferation,protein secretion and migration with minor cell apoptosis were also discussed in details,which contributed to the potential clinical application as a new biomaterial for orthopedics.
