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国家自然科学基金(81060372)

作品数:16 被引量:90H指数:7
相关作者:李旻辉张春红王振旺张娜李振华更多>>
相关机构:包头医学院内蒙古科技大学中国中医科学院更多>>
发文基金:国家自然科学基金教育部科学技术研究重点项目内蒙古自治区高等学校科学研究项目更多>>
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An ethnopharmacological investigation of medicinal Salvia plants (Lamiaceae) in China被引量:2
2013年
In China,over 40 species of the genus Salvia have been used as medicinal plants for various diseases,some for thousands of years.Recently,research has focused on the biological activities of Salvia medicinal plants used in traditional chinese medicine(TCM).However,to date a scientific survey of the genus Salvia in China has not been carried out.In this paper,we report the results of 10 field surveys of Salvia medicinal plants collected in 17 provinces including detailed information on their local names,growing environment,distribution and therapeutic effects.We also summarize the results of research on the materia medica,phytochemistry and pharmacology of some of the important Salvia medicinal plants.Our study reveals that 35 Salvia plants have been used in TCM in different regions of China,including 20 species used as Danshen to treat heart diseases,and 15 species used to treat a range of other conditions including gynecological diseases,muscular or skeletal problems,hepatitis,urological diseases,and mouth and eye conditions.It is clear that some species of Salvia L.possess significant pharmacological activity in the context of ethnopharmacological knowledge,especially in the treatment of heart disease.Further studies of the phytochemistry and pharmacology of Salvia species will no doubt improve their medical utilization and contribute to the development of new natural drugs.
Minhui LiQianquan LiChunhong ZhangNa ZhangZhanhu CuiLuqi HuangPeigen Xiao
关键词:SALVIAETHNOPHARMACOLOGYDANSHEN
Ethnopharmacological Investigation and Rapid Authentication of Mongolian Patent Medicines Digeda
2015年
Objective To investigate Mongolian medicinal plants called Digeda and the prescriptions in Inner Mongolia region and to establish a molecular method for authentication of Digeda Mongolian patent medicines(MPMs). Methods A field investigation was conducted on traditional uses of Digeda. After interviewed traditional healers in Mongolian, ethnopharmacological information of Digeda prescriptions was recorded in detail, including names, compositions, and traditional uses. And the total DNA from 10 MPMs has been amplified by three pairs of specific primers. Specific PCR products were further identified by sequence alignment with the known sequences already submitted in Gen Bank or own sequences. Results Fifteen Digeda plants and 29 Digeda prescriptions with their ethnopharmacological knowledge were collected. Ten MPM samples containing Lomatogonium rotatum, Viola philippica, and Corydalis bungeana were successfully evidenced by PCR with specific bands as raw materials. Conclusion Digeda should be further investigated in ethnopharmacology, which is a fundamental step toward developing efficacious natural drugs for various diseases. PCR amplification of specific allele is an easy and economical method, which can be used to identify highly processed MPMs and will assist in monitoring their qualities and legalities.
Le ZhangZhan-hu CuiYong-xu MuKun-hua WeiZhen-hua LiHong ZhuDa-wei YangYing-li WangPing LongChun-hong ZhangMin-hui Li
关键词:传统知识GENBANKPCR产物
Ethnopharmacological Survey of Medicinal Plants in Baotou,Inner Mongolia,China被引量:2
2012年
Objective To document the knowledge and the use of indigenous medicinal plants by traditional healers in Baotou,Inner Mongolia,China.Methods Data were collected from 112 randomly-selected interviewees using semistructured interviews in wild herbal plant collected from 2007 to 2010.The data from the interviewees were analyzed with two quantitative tools.With the informant consensus factor,the information homology was evaluated and with the fidelity level the most important species from the categories were found.Results One hundred and fifty-two species belonging to 112 genera in 48 families with medicinal values were recorded.The reported medicinal plants species were used to treat 63 kinds of diseases.And the medicinal plants in this district possessed significant potentials for their pharmacological activities in the context of ethnopharmacological knowledge,especially in the treatments of gastrointestinal,dermatological,and cardiovascular diseases.Conclusion In this work,152 medicinal plants with their ethnopharmacological information are reported.This study demonstrates that many species play an important role in healing practices among inhabitants from Baotou.More ethnopharmacological information of Mongolian medicinal plants should be gathered and documented in further studies,which is a fundamental step toward developing efficacious natural drugs for various diseases.
Min-hui LIYue LIUZhen-wang WANGZhan-hu CUILu-qi HUANGPei-gen XIAO
关键词:ETHNOPHARMACOLOGYMEDICINALINFORMANTPHARMACOLOGICALMONGOLIAN
电感耦合高频等离子体发射光谱法测定蒙药角茴香中的微量元素被引量:9
2012年
目的建立电感耦合高频等离子体发射光谱(ICP-OES)法测定蒙药角茴香中Al,As,B,Ba,Cd,Co,Cu,Fe,Hg,In,Mn,Pb,Sr,Zn等14种微量元素含量的测定方法。方法采用微波消解法前处理样品,电感耦合高频等离子体发射光谱(ICP-OES)法测定蒙药角茴香中14种微量元素含量。结果该方法检出限为0.0002~0.006μg.ml-1,各元素加标回收率在95.6%~109.26%之间,RSD小于4.46%。结论该方法可快速、灵敏、准确测定蒙药角茴香中多种微量元素含量。
苏琨马强盛振华苏燕葛尔宁李旻辉
关键词:微量元素蒙药
Effects of rare earth elements on growth and metabolism of medicinal plants被引量:9
2013年
The rare earth elements(REEs)are a set of 17 chemical elements.They include the lanthanide series from lanthanum(La)to lutetium(Lu),scandium(Sc),and yttrium(Y)in the periodic table.Although REEs are used widely in industry and agriculture in China for a long time,there has been increasing interest in application of REEs to medicinal plants in recent years.In this paper,we summarize researches in the past few decades regarding the effects of REEs on the germination of seeds,the growth of roots,total biomass,and the production of its secondary metabolites,as well as their effects on the absorption of minerals and metals by medicinal plants.By compilation and analysis of these data,we found that REEs have promoting effects at low concentrations and negative effects at comparatively high concentrations.However,most studies focused only on a few REEs,i.e.,La,cerium(Ce),neodymium(Nd)and europium(Eu),and they made main emphasis on their effects on regulation of secondary meta bolism in tissue-cultured plants,rather than cultivated medicinal plants.Advanced research should be invested regarding on the effects of REEs on yields of cultivated plants,specifically medicinal plants.
Chunhong ZhangQianquan LiMuxin ZhangNa ZhangMinhui Li
关键词:GROWTHMETABOLISM
基于DNA条形码技术鉴别4种龙胆科“地格达”类蒙药基原植物被引量:15
2012年
目的:建立一种快速﹑准确和标准化的龙胆科"地格达"类蒙药材的DNA条形码鉴别方法,为"地格达"类蒙药材的分子鉴定提供依据。方法:对蒙药地格达类药用植物进行通用引物PCR扩增,PCR扩增产物直接测序,并对序列进行分析。结果:测得4种"地格达"的rbcL,ITS,trnH-psbA和matK全长序列,4种序列长度的范围为388~940 bp,rbcL,ITS,matK序列都可以将其鉴别出来。结论:trnH-psbA序列不适合用于4种"地格达"的鉴别,ITS序列可作为地格达类蒙药材一种良好的分子标记。通过DNA条形码鉴别方法可对4种龙胆科"地格达"进行准确﹑可靠﹑有效的分子鉴别。
崔占虎李旻辉袁庆军王振旺李虔全周立社
关键词:分子鉴定DNA条形码
肋柱花种子萌发影响因素研究被引量:1
2015年
目的:通过选用不同的贮存温度和不同浓度的赤霉素溶液处理肋柱花种子,找出肋柱花种子萌发的影响因素,为肋柱花的大规模栽培和有性繁殖的生产实践提供科学指导。方法:采用培养皿萌发法,以发芽率、平均根长和种子简化活力指数为指标,考察不同的贮存温度和不同浓度的赤霉素溶液对肋柱花种子萌发的影响。结果:在贮存温度对肋柱花种子萌发影响的实验中:室外变温组、4℃冰箱组和常温对照组的发芽率分别为95.3%、10.5%和6.0%;平均根长为1.19 cm、1.15 cm和1.12 cm;种子简化活力指数为1.134 1%、0.120 8%、0.067 2%。不同浓度(25 mg·L-1、50 mg·L-1、100 mg·L-1、200 mg·L-1、500 mg·L-1、1000 mg·L-1)赤霉素浸泡后种子的发芽率分别为89.6%、91.0%、94.0%、93.3%、95.0%、90.0%;平均根长为1.12 cm、1.13 cm、1.17 cm、1.15 cm、1.18 cm、1.13 cm;种子简化活力指数为1.003 5%、1.028 3%、1.099 8%、1.072 9%、1.121 0%、1.017 0%。结论:室外变温或赤霉素浸种均能显著促进肋柱花种子的萌发。
朱虹张春红韦坤华张乐李旻辉周立社
关键词:肋柱花种子萌发
“地格达类”蒙药研究进展被引量:23
2013年
"地格达类"蒙药为蒙医常用药,具有显著的清热解毒作用,在蒙药临床应用上有着极其重要的地位。为了进一步对"地格达类"蒙药进行系统深入研究并使其能够指导临床用药,该文对"地格达类"蒙药从品种考证、化学成分、定性鉴别、药理及临床应用等方面的研究进行综述。
张春红陈苏依勒张娜龙平李振华朱虹王振旺李旻辉
关键词:地格达蒙药
分子生药学在蒙药研究中的应用被引量:11
2011年
分子生药学为生药学与分子生物学有机融合形成的新兴边缘学科,通过联系分子生药学的理论和技术在中药中的应用,分析了分子生药学在蒙药中的应用研究方向,认为它在蒙药的鉴定、蒙药药用动植物种质资源的评估及保存、蒙药药用植物活性成分的生物合成及调控,以及蒙药材的道地性及分子机制等研究方面具有新的指导意义,并为蒙药的现代化研究提供了新方法、新思路。
李虔全周立社郭兰萍李旻辉张娜袁庆军袁媛
关键词:分子生药学资源保护
“地格达类”蒙药中国扁蕾全草的化学成分研究被引量:2
2015年
目的:研究“地格达类”蒙药中国扁蕾全草的化学成分。方法:利用溶剂萃取、硅胶和葡聚糖凝胶等柱色谱方法分离纯化,通过质谱、核磁共振波谱鉴定化合物的结构。结果:从扁蕾全草中分离并鉴定了9个化合物,2种三萜类化合物(1、2),2种[口山]酮类化合物(3、9)和5种黄酮类化合物(4~8):齐墩果酸(1)、熊果酸(2)、1,7-二羟基-3,8-二甲氧基[口山]酮(3)、金圣草黄素(4)、木犀草素(5)、当药黄素(6)、金圣草黄素-7-O-β-D-吡喃葡萄糖苷(7)、木犀草素-7-O-β-D-吡喃葡萄糖苷(8)、3,7,8-三甲氧基[口山]酮-1-O-[β-D-吡喃木糖基-(1-6)]-β-D-吡喃葡萄糖苷(9)。结论:化合物1-9均为首次从中国扁蕾中分离得到,化合物7为首次从龙胆科植物中分离得到。
阿如罕赵冬冬穆永旭李振华张娜庞东卫李旻辉
关键词:龙胆科三萜[口山]酮
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