Atmospheric nitrogen deposition is at a high level in some forests of South China. The effects of addition of exogenous N and P on soil organic carbon mineralization were studied to address: (1) if the atmospheric N deposition promotes soil C storage through decreasing mineralization; (2) if the soil available P is a limitation to organic carbon mineralization. Soils (0-10 cm) was sampled from monsoon evergreen broad-leaved forest (MEBF), coniferous and broad-leaved mixed forest (CBMF), and Pinus massoniana forest (PMF) in Dinghushan Biosphere Reserve (located in Guangdong Province, China). The soils were incubated at 25°C for 45 weeks, with addition of N (NH4NO3 solution) or P (KH2PO4 solution). CO2-C emission and the inorganic N (NH4+-N and NO3?-N) of the soils were determined during the incubation. The results showed that CO2-C emission decreased with the N addition. The addition of P led to a short-term sharp increase in CO2 emission after P application, and the responses of CO2-C evolution to P addition in the later period of incubation related to forest types. Strong P inhibition to CO2 emission occurred in both PMF and CBMF soils in the later incubation. The two-pool kinetic model was fitted well to the data for C turnover in this experiment. The model analysis demonstrated that the addition of N and P changed the distribution of soil organic C between the labile and recalcitrant pool, as well as their mineralization rates. In our experiment, soil pH can not completely explain the negative effect of N addition on CO2-C emission. The changes of soil inorganic N during incubation seemed to support the hypothesis that the polymerization of added nitrogen with soil organic compound by abiotic reactions during incubation made the added nitrogen retard the soil organic carbon mineralization. We conclude that atmospheric N deposition contributes to soil C accretion in the three subtropical forest ecosystems, however, the shortage of soil available P in CBMF and PMF may also retard soil organic C mineralization.
采用野外原位试验模拟氮(N)沉降,研究了其对鼎湖山马尾松林、混交林和季风林3种森林类型土壤酸性磷酸单酯酶活性(APA)和有效磷(AP)含量的影响.在季风林中设置对照(0 kg N.hm-2.a-1)、低N(50 kg N.hm-2.a-1)、中N(100 kg N.hm-2.a-1)和高N(150 kg N.hm-2.a-1)处理,在马尾松林和混交林中只设置对照、低N和中N处理.结果表明:随着土层加深,土壤APA和AP含量降低.土壤APA在季风林中最高,而AP含量在3种林型中没有显著差异.N沉降增加对土壤APA的作用与林型有关.季风林中适度N沉降可使APA升高,且低N处理的APA(19.52μmol.g-1.h-1)最高;马尾松林和混交林中,中N处理的APA最高,分别为12.74和11.02μmol.g-1.h-1.3种林型的AP含量均在低N处理下最高,但各N处理之间的差异并不显著.土壤APA与AP含量之间呈显著正相关关系.
