Land use change significantly influences soil properties.There is little information available on the long-term effects of post-reclamation from grassland to cropland on soil properties.We compared soil carbon(C) and nitrogen(N) storage and related soil properties in a 50-year cultivation chronosequence of grassland in the agro-pastoral ecotone of Inner Mongolia.Field surveys on land use changes during the period of 1955-2002 were conducted to build a chronosequence of cropland of different ages since the conversion from grassland.The results showed that soil C and N storage,soil texture,and soil nutrient contents varied with land use types and cropland ages(P<0.01).In the 0-30 cm soil layer,the soil organic carbon(SOC) density was significantly lower in the croplands(3.28 kg C/m2 for C50 soil) than in the grasslands(6.32 kg C/m2).After 5,10,15,20,35,and 50 years of crop planting(years since the onset of cultivation),the SOC losses were 17%,12%,19%,47%,46%,and 48%,respectively,compared with the grasslands.The soil total nitrogen(TN) density of the grasslands was 65 g N/m2,and TN density of the cropland soil was 35 g N/m2 after 50 years of crop planting.Both the SOC and TN densities could be quantitatively determined by a negative exponential function of cropland age(P<0.0001,R2=0.8528;P<0.0001,R2=0.9637).The dissolved organic carbon(DOC) content,soil available potassium(AK) content,clay content,and pH value were decreased;and the soil bulk density and sand content were increased since the conversion of grassland into cropland during the 50-year period.Our results show soil nutrients were higher in grassland than in cropland.The conversion of grasslands to croplands induced a loss of soil C storage and changes of related soil properties.The reclamation time of cultivated soil(cropland age) had significant effects on soil properties in the study area.