用A lam ar b lue法对提取的侧柏叶、种皮及种子挥发油进行了抗人肺癌细胞实验,结果表明侧柏叶、种皮和种子挥发油对肺癌细胞NC I-H460有明显抑制率,分别为86.24%、47.80%和97.73%。其中以叶挥发油和种子挥发油最高。采用气-质联用法对侧柏叶、种皮和种子挥发油的化学成分进行了研究,其含有的挥发物质种类侧柏叶、种皮和种子分别为8,31和21个成分,其挥发油成分也不尽相同,侧柏叶含量最高的是α,,α4,8-四甲基-3,7环癸二烯-1-甲醇和雪松醇分别占总挥发油的27.213%和26.193%,种皮和种子挥发油中含量最高的都是雪松醇,分别占总挥发油的28.277%和26.919%。其中,对侧柏叶挥发油4℃低温下重结晶得到的雪松醇进行了鉴定,纯度达97.06%,对雪松醇抗人肺癌细胞实验结果是雪松醇对人肺癌细胞NC I-H460半致死浓度为44.98μg.mL-1。
The paper reported antimicrobial function of different trees species in woodland along the Grand Canal, Yangzhou, China, at 16:30-17:00, Sep 5, 2004. The tested result showed that in different communities of Platycladus orientalis,Osmanthus fragrans, Cedrus deodara, Albizzia julibrissin, Cinnamomum camphora had antibacterial action. The content of pollutant bacteria in the forest atmosphere (1.5～1.8 m above the land) decreased obviously,which amounted to 23.59%～99.78% compared with the control in open area and 10.08%～99.71% compared with the near communities away from {15 m} distance. There was nat antifungal (against mold) effect in general; only two tree communities for Osmanthus fragrans (decreased 40.66% compared with the near 15 m distance) and P.orientalis (decreased 24.73% than in open area) had less antifungal effect. At the same time, the antimicrobial activities were tested by the extractive volatile of foliage for more tree species in lab. The results showed that antibacterial activities to against Staphalococus aureus and Escherichia coli were the highest in P.orientalis , Manglietia yuyuanensis and C.camphora , and higher in Koelreuteria bipinnata, Viburnum macrocephalum, Metasequoia glyptostroboides, Eriobotrya japonica, Ginkgo biloba, C.deodara, Magnolia grandiflora, Sabina chinensis. Most of the tree species tested had less antifungal activities against Fusarium graminerum and Glomerella cingulata. While Ginkgo biloba, Cinnamomum camphora, S.chinensis, Elaeocarpus sylvestris and C.deodara had higher antifungal activities against G.cingulata than others. The P.orientalis, C.camphora, S.chinensis Magnolia grandiflor, G.biloba, Acer mono, Acer palmatum had higher antifungal activities against F.graminerum than others.The research results recognized that the suitable cultivated tree species in cities were P.orientalis, C.deodara, C.camphora and M.glyptostroboides.
