An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection.
＜正＞The scorpion venoms are a rich source of toxic peptides.BmK IT is an excitatory scorpionβ-toxin in Buthus m...
Renjia Yang Jun Du Yuejun Fu Aihua Liang (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan,030006,PR China)
＜正＞Gene therapy is proposed as a novel therapeutic strategy for glioma.Adenovirus-mediated delivery of the con...
Jun Du~a,Yuejun Fu~a,Jianing Wang~b,Renjia Yang~a and Aihua Liang~a (a Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,PR China b Institute of Clinical Medicine,People''s Hospital,Shiyan 442000,PR China)
