A new alkylpyrrole derivative,fusariumin A(1),was isolated from the culture broth of the fungus Fusarium sp.The absolute configuration of fuasiumin A has been established as(2'R,3'R)using a combination of RDC(residual dipolar coupling)-based NMR and DFT-supported chiroptical spectroscopy.It is worth to note that in this study without the aid of the RDC analysis,an unambiguous determination of configuration and conformation was not feasible due to the excessive conformational possibilities of this open-chain compound.
Liang-Yan LiuHan SunChristian GriesingerJi-Kai Liu
Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system of ergosterol skeleton.In addition,five new steroids,5β,6β-epoxy-3β,7α,9α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(2),5β,6β-epoxy-3β,7α-dihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(3),5α,6α-epoxy-3β,9α,15α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-7-one(4),15α-acetoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(5),15β-methoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(6),along with four known ergosterols(7-10),were obtained.All structures were elucidated based on 1D and 2D NMR spectral data.New compounds were evaluated for cytotoxicity against five human cancer cell lines,only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.
Shuang WANGLing ZHANGLiang-Yan LIUZe-Jun DONGZheng-Hui LIJi-Kai LIU
Three new sesquiterpene aryl esters,named 10-dehydroxy-melleoliede B(1),1-O-formyl-10-dehydroxy-melleoliede B(2)and 10-oxo-melleoliede B(3)together with six known ones(4-9),were isolated from the cultures of Armillaria sp.The structures of the new compounds were elucidated based on the extensive spectroscopic methods.Compounds 1,2,and 5-9 exhibited moderate cytotoxicities.
Three new humulane-type sesquiterpenes,antrodols A–C(1–3),were isolated from cultures of the fungus Antrodiella albocinnamomea.Their structures were elucidated on the basis of extensive spectroscopic analysis.Antrodols A–C(1–3)are first examples of humulane-type sesquiterpenes isolated from cultures of higher fungi,and antrodol A(1)was the first report of humulane-type sesquiterpene with a methyl rearranged at C-3.All compounds were evaluated in the enzyme inhibition assay against two protein-tyrosine phosphatases(PTPs):MEG2 and PTP1Bc.
Zi-Ming ChenQiong-Ying FanXia YinXiao-Yan YangZheng-Hui LiTao FengJi-Kai Liu
Five new 5,6-seco-tremulane sesquiterpenoids(1-5),as well as three known analogues(6-8),were isolated from the basidiomycete Conocybe siliginea.The structures of new compounds were elucidated by extensive spectroscopic methods.The known compounds were identified by comparing their spectroscopic data with those reported in the literature.
Xiao-Yan YANGTao FENGJian-Hai DINGXia YINHua GUOZheng-Hui LIJi-Kai LIU
AIM: To investigate the chemical constituents of the cultures of Laetiporus sulphureus(Bull.) Murrill. METHOD: Compounds were isolated and purified by various chromatographic techniques. The structure of the new compound was determined by interpretation of MS and 1D-, 2D-NMR spectroscopic data, while the known compounds were identified by comparison of their data with those reported. RESULTS: Three mycophenolic acid derivatives, 6-((2E, 6E)-3, 7-dimethyldeca-2, 6-dienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one(1), 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-5, 7-dihydroxy-4-methylphtanlan-1-one(2), and 6-((2E, 6E)-3, 7, 11-trimethyldedoca-2, 6, 10-trienyl)-7-hydroxy-5-methoxy-4-methylphtanlan-1-one(3) were isolated. CONCLUSION: Among them, compound 1 was new, and compound 2 exhibited moderate cytotoxicity against HL-60, SMMC-7721, A-549, and MCF-7 cells, with IC50 values of 39.1, 31.1, 27.4, and 35.7 μmol·L-1, respectively.
FAN Qiong-YingYIN XiaLI Zheng-HuiLI YanLIU Ji-KaiFENG TaoZHAO Bao-Hua
Five new guanacastane-type diterpenes,named guanacastepenes P–T(1–5),were isolated from cultures of the fungus Psathyrella candolleana.Their structures were elucidated on the basis of extensive spectroscopic methods.All of the compounds were tested for their 11b-hydroxysteroid dehydrogenase(11b-HSD1)inhibitory activity.Compound 3 exhibited inhibitory activity against both human and mouse isozymes of 11b-HSD1 with IC50 values of 6.2 and 13.9 lM,respectively.
Seven cadinane sesquiterpenoids,named boreovibrins A-G(1-7),three dihydrobenzofurans(8-10),and two lactones(11 and 12),together with one known compound(13),were isolated from cultures of the basidiomycete Boreostereum vibrans.The new structures were elucidated by means of spectroscopic methods.Compounds 5,6,and 11 showed weak inhibitory activities against isozymes of 11β-hydroxysteroid dehydrogenases(11β-HSD).
Jian-Hai DINGTao FENGZheng-Hui LILiang LIJi-Kai LIU
