目的观察女性多发性硬化(multiple sclerosis,MS)患者的性功能障碍情况。方法选取2011年6月至2017年12月首都医科大学附属北京天坛医院中医科诊治的76例MS患者作为研究对象。采用电话与电子调查问卷,结合病历资料,收集患者的人口学与临床资料、性功能状况、二便情况等数据进行统计分析。结果 76例被访患者中有53例患者的FSFI评分低于26.5分,女性性功能障碍(female sexual dysfunction,FSD)的发生率为69.7%,FSD组患者平均年龄和发病年龄均显著高于无FSD患者,年龄≥40岁的患者在FSD组所占比例(54.7%)显著高于无FSD组(17.4%),两组间病程、发病次数无显著差异,≥40岁患者FSFI调查量表总得分及性欲、性唤起、阴道润滑、性交痛4个维度单项得分均显著低于<40岁患者,49例存在大便和(或)小便异常(64.5%),Logistic逐步回归分析显示年龄和病程是女性MS患者发生性功能障碍的独立危险因素。结论女性MS患者并发性功能障碍的发生率高,影响患者生活质量。
Objective： To study the effects of Bushen Yisui Capsule（补肾益髓胶囊, BSYSC） on the oligodendrocyte lineage genes（Olig） 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis（EAE） in order to explore the remyelination effect of BSYSC. Methods： The mice were randomly divided into normal control（NC）, EAE model（EAE-M）, prednisone acetate（PA, 6 mg/kg）, BSYSC high-dose（3.02 g/kg） and BSYSC low-dose（1.51 g/kg） groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein（MOG） 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin（HE） staining and transmission electron microscope（TEM）. The protein expressions of myelin basic protein（MBP）, Olig1 and Olig2 in brains were measured by immunohistochemistry. The m RNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Results： Compared with the EAE-M mice,（1） the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40（P〈0.01）;（2） the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice;（3） the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively（P〈0.05 or P〈0.01）;（4） the protein expression of Olig1 was increased in BSYSC（3.02 g/kg）-treated EAE mice on day 40（P〈0.01）. Protein and m RNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40（P〈0.01）. Conclusion： The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.
