A new minor pregnane glycoside was isolated from the fermented leaves of Agave americana. Its structure was elucidated as (20S)-5a-pregnane-3? 20-diol 20-O--D-glucopyrano- side (1) by spectral methods.
Fifteen known compounds,cerebroside B(1),psicofuranine(2),uridic triphosphate(3),uracil(4),adenine(5),3β-acteoxy-(22E,24R)-24-methyl-5α-cholest-7,22-diene-5,6β-diol(6),(22E)-27-nor-24-methyl-5α-cholesta-7,22-diene-3β,5,6β-triol(7),3β-hydroxy-5α,8α-epidioxy-24ξ-methylcholesta-6-en(8)(22?mg),3β-O-glucopyranosyl-5α,6β-dihydroxyergosta-7,22-diene(9),(24S)-ergosta-4,6,8(14),22-tetraen-3-one(10),(22E,24R)-24-methylergosta-7,22-diene-3β,5α,6β-triol(11),(22E,24S)-24-methyl-5α-cholest-7,22-diene-3β,5,6β-triol(12).3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene (13),3β-hydroxyergosta-5,7,22-triene(14) and D-allitol(15)loene isolated from the fresh fruiting bodies of Sarcodon aspratum (Berk.) S.Ito,and their structures were identified by means of spectroscopy.All the compounds are reported firstly in genus Sarcodon ?
Parvifloside (1), a new furostanol pentaglycoside, was isolated from the fresh rhizomes of Dioscorea parviflora C. T. Ting. On the basis of spectroscopic and chemical methods, its structure was elucidated as (25R)-26-O-β-glucopyranosyl-furost-5-en-3β,22ξ,26-triol 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosyl (1→4)-[α-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside. Six prosapogenins (2-7)were obtained from the enzymatic degradation of 1by cellulase, but only 3 and 4 were obtained by β-glucosidase. The structures of all compounds were determined by spectroscopic data. The activity of the isolated compounds on deformation of mycelia germinated from Pyricularia oaryzae P-2b conidia was evaluated.
