Objective:To investigate the therapeutic effects of Qingre Quyu Granule(清热祛瘀颗粒,QQG)on the patients with severe carotid stenosis,and to explore the mechanism of it.Methods:Ninety-six patients with severe carotid stenosis were enrolled in the study and were classified into a QQG group(n=48) and a control group(n=48) randomly using consecutively numbered envelopes.The patients in the QQG group were given QQG and Western medicine,those in the control group were given Western medicine merely,the course of treatment was 16 weeks.All patients went through endarterectomy after treatment.Plaques were subjected to the analysis of CD3,CD68,soluble intercellular adhesion molecule 1(ICAM-1),matrix metalloprotease-9(MMP-9),CD40 L,tenascin-C,and collagen content lipid content by immunohistochemistry or polarized light analysis.Results:By the end of experiment,the expressions of CD3,CD68,ICAM-1,MMP9,CD40 L and tenascin-C on the plaques were statistically significant lower in the QQG group compared with the control group(P<0.01).The lipid content of the plaque was also significantly lower in the QQG group compared with the control group(P<0.01).The interstitial collagen in the tissue sections of the plaques was also significantly higher in the QQG group in comparison with the control group(P<0.01).Conclusion:QQG could stabilize carotid artery plaques through inhibiting pro-inflammation factors and restraining the tenascin-C and MMP9 pathway.
目的:探讨长期中等强度负荷耐力运动对载脂蛋白(Apo E)基因敲除小鼠主动脉粥样硬化易损斑块的影响和对主动脉基质金属蛋白酶9(MMP9)、腱糖蛋白-C(tenascin-C)表达的调控作用。方法:40只6周龄Apo E基因敲除小鼠随机分为运动干预组(20只)和对照组(20只)。运动干预组给予长期中等强度的负荷耐力跑台运动干预32周;一般对照组未予以特殊干预。实验结束后评估两组小鼠主动脉动脉粥样硬化斑块面积和动脉粥样硬化斑块脂核面积的变化情况、采用偏振光的方法测定主动脉胶原含量的变化,采用免疫组化方法测定主动脉MMP9、tenascin-C的表达。结果:长期中等强度负荷耐力运动组主动脉粥样硬化斑块面积、斑块脂核面积及埋藏纤维帽数目均显著下降[(70932±4669)μm2vs(57247±3982)μm2,P<0.01;(21279±2104)μm2vs(57247±3982)μm2,P<0.01;1.03±0.42vs 5.22±0.77,P<0.01]、主动脉壁胶原含量显著增加(67.41%±7.88%vs 21.22%±8.63%,P<0.01),主动脉MMP9(47.02±8.55 vs 36.67±4.51,P<0.01)、tenascin-C的表达显著低于对照组(39.72±6.89 vs 28.86±2.90,P<0.01)。结论:长期中等负荷耐力运动可显著下调Apo E基因敲除小鼠主动脉MMP9、tenascin-C的表达并具有稳定主动脉易损斑块的作用。
