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广东省自然科学基金(9351027501000003)

作品数:8 被引量:19H指数:3
相关作者:计亮年巢晖杜可杰王忆梁捷雯更多>>
相关机构:中山大学更多>>
发文基金:广东省自然科学基金国家自然科学基金国家教育部博士点基金更多>>
相关领域:生物学理学化学工程医药卫生更多>>

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8 条 记 录,以下是 1-10
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羧酸咔咯与DNA的相互作用
<正>DNA是生物体内重要的遗传物质,研究化合物与DNA的相互作用对DNA探针、靶向抗癌药物和人工模拟核酸酶等开发均有重要意义[1-3]。近年来,咔咯与生物大分子的相互作用研究引起了化学工作者的兴趣
闻金燕张阳王惠计亮年刘海洋
关键词:DNA
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代谢工程大肠杆菌过量生产番茄红素被引量:1
2012年
番茄红素是一种高效抗氧化剂和潜在抗癌药物。gdhA,aceE和fdhF基因敲除促进了重组大肠杆菌番茄红素的合成,其中gdhA和aceE双基因敲除和gdhA,aceE和fdhF三基因敲除具有相似的作用。在gdhA和aceE双基因敲除的基础上,dxs基因天然启动子被T5启动子置换后,重组大肠杆菌番茄红素的产量提高了103%。为了避免采用诱导剂进行基因表达,构建了一系列组成型质粒,最终构建的代谢工程大肠杆菌BW25113(rgdhAraceE, PT5-dxs,pAC316-WZM4R)在不需诱导条件下摇床发酵可产番茄红素15.6 mg/g DCW。
翁志明王玥刘建忠
关键词:番茄红素大肠杆菌代谢工程
两种钌配合物皮秒发光动力学过程研究
2011年
采用稳态发光光谱、瞬态发光动力学测量等手段,对两种钌配合物[Ru(bpy)3](ClO4)2和[Ru(bpy)2HPIP](ClO42)的发光性质进行了研究。稳态发光光谱表明[Ru(bpy)2HPIP](ClO4)2发光明显偏弱;皮秒瞬态发光动力学测量显示[Ru(bpy)2HPIP](ClO42)的激发态弛豫过程存在皮秒量级的快过程,可能与由于HPIP配体的存在,使电荷转移态和溶剂分子产生氢键作用有关。纳秒瞬态发光动力学测量的结果则显示了所有样品共同具有的瞬态发光衰减过程,实验结果符合能隙定律,推断其来自没有和溶剂分子形成氢键结合的配合物的激发态弛豫过程。
徐忠扬王丽丽倪泉丰巢晖王惠张蕾计亮年
关键词:激光光学钌配合物
Synthesis,crystal structures,electrochemical and spectroscopic properties of ruthenium(Ⅱ) complexes containing diamino-1,3,5-triazine derivatives被引量:1
2010年
Three Ru(Ⅱ) complexes [Ru(bpy)2(1-IQTNH)](ClO4)2 (1), [Ru(bpy)2(2-QTNH)](ClO4)2 (2) and [Ru(bpy)2(3-IQTNH)](ClO4)2 (3) (bpy = 2,2′-bipyridine, 1-IQTNH = 6-(isoquinolin-1-yl)-1,3,5-triazine-2,4-diamine, 2-QTNH = 6-(quinolin-2-yl)-1,3,5-triazine-2,4-diamine, 3-IQTNH = 6-(isoquinolin-3-yl)-1,3,5-triazine-2,4-diamine) have been synthesized and characterized by elemental analysis, 1H NMR spectroscopy, electrospray ionization mass spectrometry and X-ray crystallography. The electrochemical and spectroscopic properties of the complexes differ from those of [Ru(bpy)3]2+ owing to the structural differences between the ligands and their complexes.
CHEN Yu, XU WenChao, KOU JunFeng, WEI XuHui, YU BoLe, CHAO Hui & JI LiangNian MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China
关键词:ELECTROCHEMISTRY
Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q_(10)被引量:4
2011年
Escherichia coli BW25113 was metabolically engineered for CoQ10 production by replacing ispB with ddsA from Gluconobacter suboxydans.Effects of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate (NADPH) availability on CoQ10 production in E.coli were investigated.The knockout of pykFA along with pck overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate,increasing CoQ10 production.Replacement of native NAD-dependent gapA with NADP-dependent gapC from Clostridium acetobutylicum,together with the overexpression of gapC,could increase NADPH availability and then enhanced CoQ10 production.Three effects,overexpressions of various genes in CoQ biosynthesis and central metabolism,different vectors and culture conditions on CoQ10 production in E.coli,were all investigated.The investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ10 production in E.coli.The recombinant E.coli (△ispB::ddsA,△pykFA and △gapA::gapC),harboring the two plasmids encoding pck,dxs,idi and ubiCA genes under the control of PT5 on pQE30,ispA,ddsA from Gluconobacter suboxydans and gapC from Clostridium acetobutylicum under the control of PBAD on pBAD33,could produce CoQ10 up to 3.24 mg·g-1 dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to 5.5.The yield is unprecedented and 1.33 times of the highest production so far in E.coli.
黄明涛王玥刘建忠毛宗万
代谢工程联合适应性进化谷氨酸帮杆菌高效生产合成鸟氨酸
鸟氨酸是尿素循环中的重要组成成分和合成瓜氨酸和精氨酸的前体物。具有解氨毒、治肝、护肝、伤口治愈、激烈运动后的体力恢复等多种生物功能,在医疗、保健、食品等领域有重要作用。随着鸟氨酸生物功能的发现,市场对其需求逐年增加,引起...
刘建忠蒋玲艳
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Chromosomal Engineering of Escherichia coli for Efficient Production of Coenzyme Q_(10)
2014年
The plasmid-expression system is routinely plagued by potential plasmid instability. Chromosomal integration is one powerful approach to overcome the problem. Herein we report a plasmid-free hyper-producer E.coli strain for coenzyme Q10 production. A series of integration expression vectors, pxKC3T5b and pxKT5b, were constructed for chemically inducible chromosomal evolution(multiple copy integration) and replicon-free and markerless chromosomal integration(single copy integration), respectively. A coenzyme Q10 hyper-producer Escherichia coli TBW20134 was constructed by applying chemically inducible chromosomal evolution,replicon-free and markerless chromosomal integration as well as deletion of menaquinone biosynthetic pathway.The engineered E. coli TBW20134 produced 10.7 mg per gram of dry cell mass(DCM) of coenzyme Q10 when supplemented with 0.075 g·L-1of 4-hydroxy benzoic acid; this yield is unprecedented in E. coli and close to that of the commercial producer Agrobacterium tumefaciens. With this strain, the coenzyme Q10 production capacity was very stable after 30 sequential transfers and no antibiotics were required during the fermentation process. The strategy presented may be useful as a general approach for construction of stable production strains synthesizing natural products where various copy numbers for different genes are concerned.
黄明涛陈韵妍刘建忠
Engineering of Corynebacterium glutamicum to Enhance L-ornithine Production by Gene Knockout and Comparative Proteomic Analysis被引量:3
2012年
Engineered Corynebacterium glutamicum was constructed for L-ornithine production by disrupting genes of argF and proB to prevent the flux away from L-ornithine.Effect of the inactivation of 2-oxoglutarate de-hydrogenase complex(ODHC) on L-ornithine production was also investigated.It was found that the inactivation of ODHC by knockout of the kgd gene enhanced L-ornithine production.The engineered C.glutamicum ATCC13032(ΔargFΔproBΔkgd) produced L-ornithine up to 4.78 g·L-1 from 0.24 g·L-1 of the wild-type strain.In order to understand the mechanism of L-ornithine production in C.glutamicum ATCC13032(ΔargFΔproBΔkgd) and find out new strategies for further enhancing L-ornithine production,the comparative proteome between the wild-type and the engineered strain was analyzed.L-Ornithine overproduction in the engineered strain was related to the up-regulation of the expression levels of enzymes involved in L-ornithine biosynthesis pathway and down-regulation of the expression levels of proteins involved in pentose phosphate pathway.The overexpression of genes in the upstream pathway of glutamate to increase the availability of endogenous glutamate may further in-crease ornithine production in the engineered C.glutamicum and the ornithine synthesis enzymes(ArgCJBD) may not be the limiting enzymes in the engineered C.glutamicum.
卢冬梅刘建忠毛宗万
DNA拓扑异构酶抑制剂被引量:7
2013年
DNA拓扑异构酶是一种广泛存在于真核细胞和原核细胞中的重要生物酶,对DNA转录、复制、染色体分离及基因表达等过程中的DNA拓扑结构起着重要的调控作用。研究发现,与正常细胞不同,DNA拓扑异构酶在肿瘤细胞中表现出不受其他因素影响的高水平表达,而许多抗肿瘤药物的作用机制也与DNA拓扑异构酶密切相关,因此它作为抗肿瘤药物的重要靶点引起了研究者的广泛关注。本文对DNA拓扑异构酶的结构、分类及生物功能进行了简要的归纳,总结了近年来DNA拓扑异构酶抑制剂的研究进展,并重点对金属配合物作为DNA拓扑异构酶抑制剂的研究现状进行了详细的介绍。
杜可杰王忆梁捷雯计亮年巢晖
关键词:DNA拓扑异构酶生物功能抑制剂金属配合物抗肿瘤药物
具有非共价相互作用的金属酶模拟物被引量:3
2013年
金属酶催化的高效性和选择性来源于第一配位环境金属催化中心和第二配位环境非共价相互作用力的协同作用。针对金属催化中心构效关系的研究已有大量报道,相比之下,有关非共价相互作用的研究尚不够充分。金属酶的非共价作用力,包括氢键、静电相互作用、范德华力和疏水相互作用等产生于第二配位环境中的氨基酸残基。阐述第二配位环境中氨基酸残基作用的首要障碍来自于复杂并难以界定的分子内和分子间的相互作用网络。制备包含非共价相互作用的金属酶模拟物是攻克这一难题行之有效的方法,它不但有利于理解非共价相互作用和金属离子之间的协同作用,而且有助于发展可应用于工业、医药、生物技术等领域的仿生催化剂。本文按照非共价弱相互作用的类型,对近期报道的典型案例进行综述。文中阐述了基于简单多齿配体,如联吡啶、三联吡啶、环胺、卟啉等,和基于超分子配体,如功能化的环糊精和杯芳烃等的金属酶模型物。在讨论模型物之前,本文对天然金属酶中的非共价相互作用简略探讨。
王海波赵猛计亮年毛宗万
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