目的构建子痫前期患者绒毛外滋养细胞（extravillous trophoblast, EVCT）组织芯片，并利用该组织芯片检测磷酸化信号转导和转录活化因子3 （phosphorylated signal transducer and activator of transcription 3,pStat3）在子痫前期EVCT中的表达水平，探讨Stat3信号途径在子痫前期病理过程中的作用。方法收集2007年12月12日至2010年12月31日郑州大学第三附属医院80例子痫前期和58例正常妊娠孕妇的胎盘组织用来构建组织芯片，利用波形蛋白、细胞角蛋白和人类白细胞抗原-G抗体进行免疫组织化学染色验证EVCT组织芯片，采用免疫组织化学半定量方法检测pStat3在子痫前期和正常妊娠孕妇胎盘EVCT组织的表达情况。采用非参数秩和检验、Kruskai-wallisH检验、t检验和卡方检验进行统计分析。结果57例子痫前期（109个组织点）和31例正常妊娠孕妇的胎盘块（65个组织点）适于EVCT组织芯片的构建。组织芯片中EVCT细胞角蛋白染色阳性，波形蛋白染色阴性，人类白细胞抗原-G染色阳性，符合EVCT的特点，86．4％（76／88）的样本保留了EVCT，组织芯片构建成功。子痫前期患者胎盘EVCT中pStat3阳性率为51．1％（24／47），重度子痫前期组阳性率为52．3％（23／44），早发型子痫前期组阳性率为50．0％（19／38），均显著低于正常妊娠组的72．4％（21／29），差异均有统计学意义（U分别为492．00、473．00和401．00，P均〈0．05）。结论EVCT组织芯片构建成功，利用该芯片可高通量检测pStat3的表达情况。pStat3信号转导途径可能参与了早发型和重度子痫前期的发生发展。
Objective:To investigate the association between the polymorphism of HLA-A,HLA-B genes and pre-eclampsia. Methods:HLA-A,HLA-B genotyping was performed by polymerase chain reaction sequence-specific primer (PCR-SSP) in 119 preeclampsia patients,117 normal pregnant women and their neonates. Results:The study showed that 16 HLA-A and 39 HLA-B alleles were obtained in pre-eclamptic patients and normal pregnant women. 15 HLA-A and 37 HLA-B alleles were obtained in their neonates. No significant difference was found in maternal or neonatal HLA-A,HLA-B alleles between pre-eclampsia group and control group (Pc>0.05). The frequencies of HLA-A11,HLA-A24,HLA-B13,HLA-B14,HLA-B15,HLA-B52 maternal/fetus genetic assoications were significantly diffe-rent between pre-eclampsia group and control group (P<0.05). Conclusion:Some HLA-A,HLA-B maternal/fetus special bindings may be associated with the susceptibility or protective of pre-eclampsia.
