Hydrogen sulfide(H_2S)has been found to be the third most important endogenous gaseous signaling molecule after nitric oxide(NO)and carbonic oxide(CO)and plays crucial roles in living organisms and biological systems.Here we use aggregation-induced emission(AIE)of a small organic molecule(TPE-indo)to detect H_2S in both solution and living cells.TPE-indo can target mitochondria and aggregate to fluoresce,which can serve as a sensor for monitoring H_2S in the mitochondria.We regulate the fluorescence of AIE molecules by tuning the viscosity of the solution to form TPE-indo nanoparticles,constructing a probe for H_2S with good selectivity and high sensitivity.The nucleophilic addition of HS-to the TPE-indo is crucial for the rapid H_2S detection.The imaging and analysis of H_2S in mitochondria of living cells with the probe demonstrate potential biological applications.
Yi ZhangXianhong HuangWenwen LiuGuanxin ZhangDeqing ZhangXingyu Jiang
