The spectrum of a class of fourth order left-definite differential operators is studied. By using the theory of indefinite differential operators in Krein space and the relationship between left-definite and right-definite operators, the following conclusions are obtained： if a fourth order differential operator with a self-adjoint boundary condition that is left-definite and right-indefinite, then all its eigenvalues are real, and there exist countably infinitely many positive and negative eigenvalues which are unbounded from below and above, have no finite cluster point and can be indexed to satisfy the inequality …≤λ-2≤λ-1≤λ-0〈0〈λ0≤λ1≤λ2≤…
