Traditional sequence analysis depends on sequence alignment. In this study, weanalyzed various functional regions of the human genome based on sequence fea-tures, including word frequency, dinucleotide relative abundance, and base-basecorrelation. We analyzed the human chromosome 22 and classified the upstream,exon, intron, downstream, and intergenic regions by principal component analysisand discriminant analysis of these features. The results show that we could clas-sify the functional regions of genome based on sequence feature and discriminantanalysis.
