The polysaccharides,FIII-2,FIII-2-a,FIII-2-b and FIII-2-c were purified from the fruit body of Ganoderma tsugae(G.tsugae) to study the effects of the four polysaccharides on the productions of human proinflammatory cytokines,interleukin-1α(IL-1α) and tumor necrosis factor α(TNFα).The results show that the polysaccharides up-regulated the production of IL-1α and TNFα in THP-1 cells under non-stimulatory condition or under stimulation of both lower concentration lipopolysaccharide(LPS) and phorbol myristate acetate(PMA),and they down-regulated the secretion of IL-1α and TNFα under stronger stimulatory conditions.The four polysaccharides promoted the expression of TNFα mRNA.Production of TNFα protein reflects the quantity of TNFα mRNA.These polysaccharides purified from Ganoderma tsugae bodies exhibit bidirectional regulation effects on cytokine production under different cell stimulatory conditions.
LIU Ai-dongZHENG Ke-yanMIAO Qing-fangIKEJIMA TakashiZHANG JieFEI Xiao-fang
To explore the effects of traditional herbal medicine Ganoderma tsugae(G.tsugae) on immunomodulatory and antitumor activities,the crude polysaccharides of G.tsugae were purified by filtration,diethylaminoethyl(DEAE) sepharose-fast flow chromatography and sephadex G-100 size-exclusion chromatography.Two main fractions,protein-containing glycans CSSLP-1 and CSSLP-2,were obtained via the gradient elution.The protein content,molecular weight,and monosaccharide composition of the two fractions were analyzed.Furthermore,the influence of the protein-containing glycans from G.tsugae on the activation of human acute monocytic leukemia cell line(THP-1) and their antitumor activities to the human hepatocellular liver carcinoma cell(HepG-2) in vitro were evaluated.The results indicate that CSSLP-1 and CSSLP-2 could increase the pinocytic activity of THP-1 cells and induce THP-1 cells to produce the cytokines of TNFα and IL-2,significantly.CSSLP-1 and CSSLP-2 also played an inhibiting effect on the cancer cell(HepG-2).Moreover,the anti-proliferation activity of CSSLP-1 and CSSLP-2 increased with the participation of TNFα and IL-2 or other antitumor factors induced from THP-1 cells by G.tsugae protein-containing glycan fractions.
